Sequences from confirmation amplicon


Confirmation sequences for line 140G07 and hit At4g18440 are shown.
Line ID 140G07
Locus At4g18440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10187941
Primers used for PCR 23ra - 8474, show primer details
   
Sequence Name 15-K115950-0022-140-G07C-23ra
GenBank Accession LN493122 [GenBank]
Sequencing primer gene specific primer, 23ra: 5'-GAATTGGCGATAAATTGATATGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K115950-0022-140-G07C-23ra
TGTCGAATTTACGGCGTTATCGCCTTTGGATGGACGTTATTGGAGTAAAGTTAAGGACTT
GGCTTCTTCTTTGAGCGAGTTTGGATTGATCTATTTCCGAGTTTTTGTCGAGGTAAGTGA
AGGGTTATGATCTGATTGGTTGATTGATTGAGAAGATTGTACTAGTTATGTCATGCTATA
TCTCTTTGTACGAGTAGTTTAGTTGTCTGGTCTAGTGTACTCATTTGCCTAAACTTTGAT
ATTTTTATATATTTGTTAGATCAAATGGCTTCTTAAGCTTTCGAATATTCCTGAAGTCAC
TGAAGTTCCAAGCTTTAGCAAAGAAGCTCAGAGTTTCTTGCAAGGCATAATCGATGGATT
TAGCATAGACGATGCATTGGAAATTAAGAAGATTGAGAGAGTAACAAATCATGATGTGAA
AGCAGTGGAGTATTTCTTGAAACAAAAGTGTGAATCACAACCAGAGATTGCTAAGGTTTA
GGTTCTTTTCTCTCACTTATAAGTTCTTAGGGTTTGGATGCTTTGATGCCCTGTAACTAA
AATTTTGGATGTCTTTTTACTGAGTTAGGTTCTTGAGTAAATGGCAATCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 05-K115949-0022-140-G07C-8409
GenBank Accession LN488492 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115949-0022-140-G07C-8409
ATTTACTCAGAACCTAACTCAGTAAAAAGACATCCAAAATTTTAGTTACAGGGCATCAAA
GCATCCAAACCCTAAGAACTTATAAGTGAGAGAAAAGAACCTAAACCTTAGCAATCTCTG
GTTGTGATTCACACTTTTGTTTCAAGAAATACTCCACTGCTTTCACATCATGATTTGTTA
CTCTCTCAATCTTCTTAATTTCCAATGCATCGTCTATGCTAAATCCATCGATTATGCCTT
GCAAGAAACTCTGAGCTTCTTTGCTAAAGCTTGGAACTTCAGTGACTTCAGGAATATTCG
AAAGCTTAAGAAGCCATTTGATCTAACAAATATATAAAAATATCAAAGTTTAGGCAAATG
AGTACACTAGACCAGACAACTAAACTACTCGTACAAAGAGATATAGCATGACATAACTAG
TACAATCTTCTCAATCAATCAACCAATCAGATCATAACCCTTCACTTACCTCGACAAAAA
CTCGGAAATAGATCAATCCAAACTCGCTCAAAGAAGAAGCCAAGTCCTTAACTTTACTCC
AATAACGTCCATCCAAAGGCGATAACGCCGTTAAATTCGACATCTCCAAGTCTCTAGAGC
TCACTCCATCCATTGCCGTCACCTAAAGCATATCAATTACGGCCCCAATT

Last Updated on 06.12.2018 16:11