Sequences from confirmation amplicon


Confirmation sequences for line 141A01 and hit At5g22490 are shown.
Line ID 141A01
Locus At5g22490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7460566
Primers used for PCR 02sb - 3144, show primer details
   
Sequence Name 36-K116218-0022-141-A01C-02sb
GenBank Accession LN502360 [GenBank]
Sequencing primer gene specific primer, 02sb: 5'-TACCCTTCAGGGGTGTATCTGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K116218-0022-141-A01C-02sb
ATCCCTAGGGTATTCCATATCAATCTCACTGTGGAACAAACCACAAGAACCAATCTAAAG
AATGAGAACCCGCGTCTATGGTTGTAAGATTCACGACACTTTGGGACAGGGAGGGTAGAA
AATGCCTCGGGTTCCGATGTTTTTCGGGTAGATGCTAGTATGAGAGACAAGATAGACACT
CCATCTCCTAATGCATGGTGACATCTTATGACACATATAGCTTCGGCGTCAGAGGTTTTA
GCGTTTAGGATATGAACTTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATC
TGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGT
CTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAAT
CCAC
   
Sequence Name 12-K116217-0022-141-A01C-3144
GenBank Accession LN491737 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116217-0022-141-A01C-3144
CAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGAT
CAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAGTTCATATCCTAAAC
GCTAAAACCTCTGACGCCGAAGCTATATGTGTCATAAGATGTCACCATGCATTAGGAGAT
GGAGTGTCTATCTTGTCTCTCATACTAGCATCTACCCGAAAAACATCGGAACCCGAGGCA
TTTTCTACCCTCCCTGTCCCAAAGTGTCGTGAATCTTACAACCATAGACGCGGGTTCTCA
TTCTTTAGATTGGTTCTTGTGGTTTGTTCCACAGTGAGATTGATATGGAATACCCTAGTG
GATTCCTTTTTGTGCATGGCAACAATATTTTTCTTGAAGGATACAGATACACCCTGGAAG
GGT

Last Updated on 06.12.2018 16:11