Sequences from confirmation amplicon


Confirmation sequences for line 141B10 and hit At1g55110 are shown.
Line ID 141B10
Locus At1g55110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20562034
Primers used for PCR 12de - 8474, show primer details
   
Sequence Name 51-K109676-0022-141-B10C-12de
GenBank Accession LN507531 [GenBank]
Sequencing primer gene specific primer, 12de: 5'-GAGATCAAGCAAGTGTGTCTTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K109676-0022-141-B10C-12de
ACATTAATCGCATCATCAAGAACAGTGTTTTGTTCCCAATCGTCTCTTAAGAGGAAGAGA
AATCAACCAGGAAATCCAGGTTTGTTTCTTTTGATAGTCTTGATGATGAAGAAGAAGAAA
AATCAACTTTTTTTTTTGTTTTTTTTGGTTGGGACGTATCCATAATAACCACCGATTATG
GTTTTTAGAATTAGGGGTCCCCCCCAAATATCCTTTTTTCCCCTTTTAACTTTGCCTCCT
ATCCCACCTCTTTAATTACAAGGGCCAAAACCCAAATAAAAATCCAAACTTTATTGGGAA
ATCTCTTATTCTAATAGGGGTTAAGTTAAATCTTCTTTTCAGGCACATTTCAGTTATAAC
CGGATTAAGTTTGGGACTTTATTGGGGGGGTTTTTTCTTCAAACCCAAAAGCAAAAGTGA
TGGCCTTATCCCCAAAGACCCTAATGGCGACAAAATGAGTATGATGGCGATATATTCAAT
TGTAAATGGCTCCATGTCCGGGAAATCTACTTGGATCAGCAATGAGTATGATGGTCAATT
TGGAGAAAAAGAAAGAGTAATTCCCATTTTTTTTTCAATTCAAAAAGGTAAA
   
Sequence Name 35-K109675-0022-141-B10C-8409
GenBank Accession LN501865 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K109675-0022-141-B10C-8409
GCCATTTACAATTGAATATATCGCCATCATACTCATTTTGTCGCCATTAGTGTCTTTGGT
GATAAGGCCATCACTTCTGCTTCTGGGTCTGAAGAAAAAACACACCAAATAAAGTCCAAA
ACTTAATCAGGTTATAACTGAAATGTGCTTGAAAAGAAGATTTAACTTAACCCATATTAG
AATAAGAGATTTCCAAATAAAGTTTGGATTTTTATTTGGTTTTTGACCATTGTAATAATA
GAGATGAGATAGAAGACAAAGTTAAAAGGGTAAAAAAGAATATTCGGAGAGTACCCATAA
TTCTAAAAACAATAATCGATGATTATTATGAATACGTCCAAACAAAAAAAAACAAAAAAA
AAAGTTGATTTTTCTTCTTCTTCATCATCAAGACTATCAAAAGAAACAAACCGGGATTTC
CGGGTTGATTTCTCTTCCTCTTAAAAGACAATGGTGGAACAAAACACTGTTCTTGATGAT
GCTGATAAAGGTTAAAGCCACTAGTTTCAGTTCTGTTTCCGGAAAACACACTTGCTTGAT
C

Last Updated on 06.12.2018 16:11