Sequences from confirmation amplicon


Confirmation sequences for line 141D01 and hit At1g15930 are shown.
Line ID 141D01
Locus At1g15930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5471732
Primers used for PCR 09cv - 8474, show primer details
   
Sequence Name 34-K109136-0022-141-D01C-09cv
GenBank Accession LN501448 [GenBank]
Sequencing primer gene specific primer, 09cv: 5'-TAGGAGGATAAACTGAGCGACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K109136-0022-141-D01C-09cv
GAGGAAACTAGACTTACACCAGCCCATTCGCCAAGGGTCTTGGCACTTGGAACTGTTAGC
AACCTGACTTCGTGATCAGCACAGAGAGCCTTCACAAGCTTGACATAATCAGGCTGGTTG
CAGTCTTCAGCCAAGACAACGAGTTGAGCCACACGCTTCTCAATAAGCTTAGCACACTCA
TGGAGACCACGAACAACACCACCATAAGCACGAGCTTTCCTAAGAGTCAACTCCAATGCA
GTCATTAAGTCCATGTCCTCTGGGATGGCCGCTGGCTCAGCAACGGGGGGAGGAACAACA
ACAGGGGCAGCCTCATCACTGAAAAGTCAGGACACAACACATGATATCATATACTTGGAT
TGTCCACAATAAACATCGAAACATCTAAAGCATGGAACAATTCTGATTTGAAACTTGTCA
AGAACCAACTAGACGCGATTAGAAGAATCAGACAAGCTAAACAAACAAAATGATACTCGT
ACAGCAACTTTCTGATTATTCCGAAGCTTGACTAAGTCCCACAAAACAGAAATCAACAAT
GTAACAACAGATGCTATTTAATTAAATAGATAAGGCCACCTCAAAATAGAAGAGAGAATT
ATCGTGGAGTATATGAAAAAGGGATGGATAATCAGAGAAATTAGCTACATGTATTACTGA
TAACGCAAGATTCACAAATGCAGTTAACAACGAAGGAGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCC
   
Sequence Name 37-K109135-0022-141-D01C-8409
GenBank Accession LN502645 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K109135-0022-141-D01C-8409
ACTGCATTTGTGATCTTGCGTTATCAGTAATACATGTAGCTAATTTCTCTCATTATCCAT
CCCTTTTTCATATACTCCACGATAATTCTCTCTTCTATTTTGAGGTGGCCTTATCTATTT
AATTAAATAGCATCTGTTGTTACATTGTTGATTTCTGTTTTGTGGGACTTAGTCAAGCTT
CGGAATAATCAGAAAGTTGCTGTACGAGTATCATTTTGTTTGTTTAGCTTGTCTGATTCT
TCTAATCGCGTCTAGTTGGTTCTTGACAAGTTTCAAATCAGAATTGTTCCATGCTTTAGA
TGTTTCGATGTTTATTGTGGACAATCCAAGTATATGATATCATGTGTTGTGTCCTGACTT
TTCAGTGATGAGGCTGCCCCTGTTGTTGTTCCTCCCCCCGTTGCTGAGCCAGCGGCCATC
CCAGAGGACATGGACTTAATGACTGCATTGGAGTTGACTCTTAGGAAAGCTCGTGCTTAT
GGTGGTGTTGTTCGTGGTCTCCATGAGTGTGCTAAGCTTATTGAGAAGCGTGTGGCTCAA
CTCGTTGTCTTGGCTGAAGACTGCAACCAGCCTGATTATGTCAAGCTTGTGAAGGCTCTC
TGTGCTGATCACGAAGTCAGGTTGCTAACAGTTCCAAGTGCCAAGACCCTTGGCGAATGG
GCTGGTGTAAGTCTATGTTTTCCTCTTTAATACTGACTCTTCATATATACCTTATTGTCG
CTCAGTTTATCCTCC

Last Updated on 06.12.2018 16:11