Sequences from confirmation amplicon


Confirmation sequences for line 141D06 and hit At3g17570 are shown.
Line ID 141D06
Locus At3g17570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6009502
Primers used for PCR 05qn - 8474, show primer details
   
Sequence Name 82-K115691-0022-141-D06C-05qn
GenBank Accession LN514227 [GenBank]
Sequencing primer gene specific primer, 05qn: 5'-CGTCTTCTAGGGTTTAACTGATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K115691-0022-141-D06C-05qn
TCGGCTAGTAGGTTTCGGTTTTTGGTTCAGTTTTGATCCAGTTTCCTCCATAGTTGTGAT
AATGAGTTTTAATCGTTGTTTCATTGCTTTTGTATCTGCTTTCTCCAAGTTGTTAGTGTT
AAAGCCCTAATTAATATCTAACATACTGTTGCTTTCTTTCTTATGTAAAATCAGGTCAGA
AACCACAAGACAAACAAACAAGGATGTTTACCGATCTTCCACGAGATTTAGAAACAGAAA
TACTCTCAAGAGTTCCCGCCACATCTCTACAAAAATTGAAGCCTACTTGCAAACGGTGGT
CAATATGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 34-K115690-0022-141-D06C-8409
GenBank Accession LN501540 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K115690-0022-141-D06C-8409
TTTGCAGTAGGCTTCAATTTTTGTAGAGATGTGGCGGGAACTCTTGAGAGTATTTCTGTT
TCTAAATCTCGTGGAAGATCGGTAAACATCCTTGTTTGTTTGTCTTGTGGTTTCTGACCT
GATTTTACATAAGAAAGAAAGCAACAGTATGTTAGATATTAATTAGGGCTTTAACACTAA
CAACTTGGAGAAAGCAGATACAAAAGCAATGAAACAACGATTAAAACTCATTATCACAAC
TATGGAGGAAACTGGATCAAAACTGAACCAAAAACCGAAACCTAACTAGCCGAATAATAT
ATAATCGAAGCATTGGAGATCAAGGCGATCAGTTAAACCCTTAGAAGACG

Last Updated on 06.12.2018 16:11