Sequences from confirmation amplicon


Confirmation sequences for line 141F04 and hit At1g76185 are shown.
Line ID 141F04
Locus At1g76185 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28590654
Primers used for PCR 11op - 8474, show primer details
   
Sequence Name 11-K114972-0022-141-F04C-11op
GenBank Accession LN491237 [GenBank]
Sequencing primer gene specific primer, 11op: 5'-TGCTTTGTGATTTTCATACAGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K114972-0022-141-F04C-11op
GCAGTTGCACCGCCTGAAGTACAGGATACAGCTGAAACACCACGAGTGCTAACATCACTA
GTTCCCATATGACAAAGACTACAACATCAACCCTGAATCAAAAGCAAACGAAACGAATAG
CATAAGTACCACTATAGAATGTTCATTGTCAAAATTTAACAATAGAATCCAGATTGCAAC
AGAGTCGATAAGAATGAAAAGTGTATGTCAAATTGTCAAAGATGGTTCAACGTGGACAAG
TCAAAATTATAGTTCCTTGTGTAGGATTCACATAGATATCCAATGTTCAACGACAATCCC
TTAAAAGATGAGAAATTTACGGTTCCAATTGAATTAAATATCACAAAGTACAATTTTTCA
AATACAACTCTAAGCACGCAGCTCCAATAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 02-K114971-0022-141-F04C-8409
GenBank Accession LN487151 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114971-0022-141-F04C-8409
CATTTTTGGAGCTGCGTGCTTAGAGTTGTATTTGAAAAATTGTACTTTGTGATATTTAAT
TCAATTGGAACCGTAAATTTCTCATCTTTTAAGGGATTGTCGTTGAACATTGGATATCTA
TGTGAATCCTACACAAGGAACTATAATTTTGACTTGTCCACGTTGAACCATCTTTGACAA
TTTGACATACACTTTTCATTCTTATCGACTCTGTTGCAATCTGGATTCTATTGTTAAATT
TTGACAATGAACATTCTATAGTGGTACTTATGCTATTCGTTTCGTTTGCTTTTGATTCAG
GGTTGATGTTGTAGTCTTTGTCATATGGGAACTAGTGATGTTAGCACTCGTGGTGTTTTC
AGCTGTATCCTTGTACTTCAGGCGGTTGCAACTTGCTTTTATCCTGCTTTGTGTCACCTT
ACTATTACTCCTCTGTATGAAATCNACAAAGCA

Last Updated on 06.12.2018 16:11