Sequences from confirmation amplicon


Confirmation sequences for line 141G05 and hit At2g32260 are shown.
Line ID 141G05
Locus At2g32260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13697596
Primers used for PCR IN02 - 8474, show primer details
   
Sequence Name 21-K024207-022-141-G05C-IN02
GenBank Accession LN495498 [GenBank]
Sequencing primer gene specific primer, IN02: 5'-TACCATTCAAAAGTCATGGACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K024207-022-141-G05C-IN02
AGGGCCACTGAGAGACAACCTTGGACTTGGTCACTGGCTCACTTAGCAATATTCAGTTAT
TCACTCTGTTGTATATAACTCACCAATTAAATTCAAACAACTAATTCAAAACATTATACT
ATAGCTTTTCATTAAAAAAATTCCAAAACATTCATTTAATTATATAAACCAAAGAACAGC
TTTTAAGACTTAAAATATTTCCTAAGTATCCAACAAGTCAATACAGATTTTTTAAGAAAA
CTAAACCATTTTTTCAACTTTACAACAAAAACACCAACTGTTACAAAAAAACTCTCGAAT
TTCCTATTTCTCCAGCCTTATGACAAAGATATCAGATTAATAAAATTTAGAATTCATTAC
TTTTTCTTCATTTTTAAAATTATCTACATACTATTTATTTCTCTCCATTTTATTCAGTGA
GAAAATAAAATTACAAATGCCTGAACACAAAAATAAATAAAATTAGAATAATCAGTTTCC
TCTAGAGGATATTCTTCGTCACAAAATTAAAAAAAAAAAGAGTAGGAGAAGAGGGAAGCG
ACTAGCACCTTTTGTAGTTTTCCGATGAA
   
Sequence Name 22-K024207-022-141-G05C-8409
GenBank Accession LN495945 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K024207-022-141-G05C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCTCCTTCATCGGAAAA
CTACAAAAGGTGCTAGTCGCTTCCCTCTTCTCCTACTCTTTTTTTTTTTAATTTTGTGAC
GAAAAATATCCTCTAGAGGAAACTGATTATTCTAATTTTATTTATTTTTGTGTTCAGGCA
TTTGTAATTTTATTTTCTCACTGAATAAAATGGAGAGAAATAAATAGTATGTAGATAATT
TTAAAAATGAAGAAAAAGTAATGAATTCTAAATTTTATTAATCTGATATCTTTGTCATAA
GGCTGGAGAAATAGGAAATTCGAGAGTTTTTTTGTAACAGTTGGTGTTTTTGTTGTAAAG
TTGAAAAAATGGTTTAGTTTTCTTAAAAAATCTGTATTGACTTGTTGGATACTTAGGAAA
TATTTTAAGTCTTAAAAGCTGTTCTTTGGTTTATATAATTAAATGAATGTTTTGGAATTT
TTTTAATGAAAAGCTATAGTATAATGTTTTGAATTAGTTGTTTGAATTTAATTGGTGAGT
TATATACAACAGAGTGAATAACTGAATATTGCTAAGTGAGCCAGTGACCAAGTCCAAGGT
TGTCTCTCAGTGGCCCTCAAGGTTAACGAAGGTTGTCCATGA

Last Updated on 06.12.2018 16:11