Sequences from confirmation amplicon


Confirmation sequences for line 141G12 and hit At5g24670 are shown.
Line ID 141G12
Locus At5g24670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8450871
Primers used for PCR BJ74 - 8474, show primer details
   
Sequence Name 08-K104856-0022-141-G12C-BJ74
GenBank Accession LN489721 [GenBank]
Sequencing primer gene specific primer, BJ74: 5'-TCAAGTATATGCATCATCTGCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K104856-0022-141-G12C-BJ74
CCCTTGAGGAAACAGGGGAAATATGTTTAAATGACACACTTGAAAAACAGAATGGTTCAT
TGTCTGCTCTTTCTTGTCTGAATCCCTGGCAATGGAGTTTGCAGCCGCATGACACTGAAA
ATTGTAGCCAGTGGCATCCTCTTAGGCATGCTTCCATGGTTGCCATTGAATCCTCTTCTG
CCAGAGATAGAAATCTGTTTCCCAATCCATCCAAGATTTTTGATCAGGATCATGTTCCGC
CCTCAAATACAGATTCTCCGGCTAAAAAGCAGAAAACAAGCAGTCAGAGTCCAGACGTAA
GTTCATTCCAACCAAGTTTTTTAATTTCCTCCACATGCTTTGACTAGTACAGCATTCTCT
CTTACACTTCTGAGTTATCGATAGGTCCAAAATGACAGCAGAGAAGAGACTGTTAGAGAT
CCTTCAATGGAAAGGCCGTACCTCTGCACTGGTTATGACATTTTCCTCCTGTTGGAGCCT
TGTACAATGTGAGTTTCTACATACATATTACCCTTTTAAACTCTGCAAGTGTGTGTATCT
AATAAAGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATT
   
Sequence Name 24-K104857-0022-141-G12C-8409
GenBank Accession LN496982 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K104857-0022-141-G12C-8409
GCCATTTACATTGAAATATCCTGACTTTATTAGATACACACACTTGCAGAGTTTAAAAGG
GTAATATGTATGTAGAAACTCACATTGTACAAGGCTCCAACAGGAGGAAAATGTCATAAC
CAGTGCAGAGGTACGGCCTTTCCATTGAAGGATCTCTAACAGTCTCTTCTCTGCTGTCAT
TTTGGACCTATCGATAACTCAGAAGTGTAAGAGAGAATGCTGTACTAGTCAAAGCATGTG
GAGGAAATTAAAAAACTTGGTTGGAATGAACTTACGTCTGGACTCTGACTGCTTGTTTTC
TGCTTTTTAGCCGGAGAATCTGTATTTGAGGGCGGAACATGATCCTGATCAAAAATCTTG
GATGGATTGGGAAACAGATTTCTATCTCTGGCAGAAGAGGATTCAATGGCAACCATGGAA
GCATGCCTAAGAGGATGCCACTGGCTACAATTTTCAGTGTCATGCGGCTGCAAACTCCAT
TGCCAGGGATTCAGACAAGAAAGAGCAGACAATGAACCATTCTGTTTTTCAAGTGTGTCA
TTTAAACATATTTCCCCTGTTTCCTCGAAGGGCCTGGTCTCTGCGCTAGTCATGTCACGA
GGAGCAGAT
Primers used for PCR BJ73 - 3144, show primer details
   
Sequence Name 02-K104856-0022-141-G12C-BJ73
GenBank Accession LN487024 [GenBank]
Sequencing primer gene specific primer, BJ73: 5'-GAGATGCATTATTTCATGTGAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K104856-0022-141-G12C-BJ73
ACACAAGTTTCTGACTAGAGAGTACTGAGCCATACACAGTAATAGAAGTCGAAAGTTTTT
GGTCTACATAATAATTCAATAAGATTCCAGATTCTTGGAATGAACAAGAACAAAGAATTA
GCTTTCTCACCCAGAACCTGATTATCAATCGACTACAACAATGCATTAGACCGTGGTCAT
TTGTCTAAGTGCGTCATCAGGCAGCAAAACTCTAAACACTGCATAATGATGGTTCAAACT
CTTTTCCCCTTGAAGTCTATGAACACTCCCGAGACCACCTGCCGTGGTGTTTGGAAAAGC
ATAGAAAATCCGTTTTATTCTTTGATGCACAAGCGCCATAGGTACACACACTTGCAGAGT
ACCCTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCT
CTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTG
TGCGTCATCCCTTACGTCAGTGGAGAT
   
Sequence Name 18-K104857-0022-141-G12C-3144
GenBank Accession LN494296 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K104857-0022-141-G12C-3144
TTCGCAGACCCTTCCTCTAATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGGGTACTCTGCAAGTGTGTG
TACCTATGGCGCTTGTGCATCAAAGAATAAAACGGATTTTCTATGCTTTTCCAAACACCA
CGGCAGGTGGTCTCGGGAGTGTTCATAGACTTCAAGGGGAAAAGAGTTTGAACCATCATT
ATGCAGTGTTTAGAGTTTTGCTGCCTGATGACGCACTTAGACAAATGACCACGGTCTAAT
GCATTGTTGTAGTCGATTGATAATCAGGTTCTGGGTGAGAAAGCTAATTCTTTGTTCTTG
TTCATTCCAAGAATCTGGAATCTTATTGAATTATTATGTAGACCAAAAACTTTCGACTTC
TATTACTGTGTATGGCTCAGTACTCTCTAGTCAGAAACTTGTGTTTTAGAGAAACAACAG
AATGCAAGAAACGAACCGTCACTAACCAATCCTCAC

Last Updated on 06.12.2018 16:11