Sequences from confirmation amplicon


Confirmation sequences for line 142A08 and hit At3g62720 are shown.
Line ID 142A08
Locus At3g62720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23201918
Primers used for PCR CV18 - 8474, show primer details
   
Sequence Name 66-K030838-022-142-A08C-8409
GenBank Accession LN511520 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K030838-022-142-A08C-8409
CATTTACATTGGTAATTGCTTTCTTTTTCTGGGCTGGATTCTATGGTGGATGGATAGTGA
TGCCATGTTCACGGACATGGTGTTCGAGCTTCCATGGGAGAGGTACAAAGATTACAACTT
GGTGATGCATGGTTGGAACGAGATGGTTTATGACCAGAAGAATTGGATTGGTCTCAACAC
GGGAAGTTTCTTGCTCAGGAACTCACAGTGGTCGCTTGATCTTCTTGACGCTTGGGCTCC
TATGGGCCCAAAAGGGAAGATCCGAGAAGAAGCGGGTAAAGTCTTGACCCGGGAACTTAA
AGACCGACCCGCTTTCGAAGCTGACGATCAATCGGCGATGGTTTATCTGCTGGCGACGGA
GAGAGAGAAATGGGGAGGCAAAGTTTATCTAGAGAGTGGTTATTACTTGCACGGTTATTG
GGGGATTTTGGTAGACCGGTACGAGGAGATGATTGAGAATCATAAACCGGGTTTTGGAGA
CCATCGGTGGCCATTGGTTACGCATTTCGTCGGGTGTAAACCGTGCGGGAAATTTGGAGA
TTATCCGGTGGAACGGTGTCTACGGCAGATGGATAGAGCGT
   
Sequence Name 67-K030838-022-142-A08C-CV18
GenBank Accession LN511729 [GenBank]
Sequencing primer gene specific primer, CV18: 5'-TGTCTCCGAAATTAAACGCTCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K030838-022-142-A08C-CV18
GATATCTCCAATTTCCCGCACGGTTTACACCCGACGAAATGCGTAACCAATGGCCACCGA
TGGTCTCCAAAACCCGGTTTATGATTCTCAATCATCTCCTCGTACCGGTCTACCAAAATC
CCCCAATAACCGTGCAAGTAATAACCACTCTCTAGATAAACTTTGCCTCCCCATTTCTCT
CTCTCCGTCGCCAGCAGATAAACCATCGCCGATTGATCGTCAGCTTCGAAAGCGGGTCGG
TCTTTAAGTTCCCGGGTCAAGACTTTACCCGCTTCTTCTCGGATCTTCCCTTTTGGGCCC
ATAGGAGCCCAAGCGTCAAGAAGATCAAGCGACCACTGTGAGTTCCTGAGCAAGAAACTT
CCCGTGTTGAGACCAATCCAATTCTTCTGGTCATAAACCATCTCGTTCCAACCATGCATC
ACCAAGTTGTAATCTTTGTACCTCTCCCATGGAAGCTCGAACACCATGTCCGTGAACATG
GCATCACTATCCATCCACCATAGAATCCAGCCCAGAAAAAGAAAGCAATTACCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTA
Primers used for PCR CV17 - 8474, show primer details
   
Sequence Name 70-K030839-022-142-A08C-8409
GenBank Accession LN512304 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K030839-022-142-A08C-8409
GCATCGAGCACGCCATGTTGTAGAAGATCTCGATTCCGTGTATTCTACAGTAATCGATTT
TGTTCTTAATCGATTTCAAGAGGTAATGGTCTCCTACAGGATTCTCACACGGTTTAGGAG
CTGAACCTGTGACAAGAAGAACCCTAGGCTTGTTTGGCGCCACGAAATTAGGGAAGCTAG
GGTTTTGTTTGAGCCAATCACGTCTCTGCTCATCCCAATCAGAGATCTTGGGACCAAGAG
AATAAGGTTTATTAGTCCGGTCTCGAGATTTCTCTTCGTCTCCTTCATCAACGAATAGCT
TGTTGATATCGAAGGTCTCGTAGCTGTTACCACCATTTCCTCCGTCTTCGGACGACGTCG
TTTTGGAAGAGACCTCGACGAGGACACGGTGAGGCTCGCCGCGTTTACGCGTGTAGAAGA
AATGCTCACGGATCTCCTCGATATCTTTCTCCGGCGTACCAAACTTACCGGCTCCGATTG
TGCCACGTAAGACGATGACGGTGAGAACGAGACAAAGAATCGTCACTTTCCCTTGACGCA
TGAATCTCTGTAATCTCCGAAACCGATGCGCTCCTATACACTTCTC
   
Sequence Name 71-K030839-022-142-A08C-CV17
GenBank Accession LN512486 [GenBank]
Sequencing primer gene specific primer, CV17: 5'-CTGCGTGAAGATGATAGAGAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K030839-022-142-A08C-CV17
GAGATTCATGGCGTCAGGGAAAGTGACGATTCTTTGTCTCGTTCTCACCGTCATCGTCTT
ACGTGGCACAATCGGAGCCGGTAAGTTTGGTACGCCGGAGAAAGATATCGAGGAGATCCG
TGAGCATTTCTTCTACACGCGTAAACGCGGCGAGCCTCACCGTGTCCTCGTCGAGGTCTC
TTCCAAAACGACGTCGTCCGAAGACGGAGGAAATGGTGGTAACAGCTACGAGACCTTCGA
TATCAACAAGCTATTCGTTGATGAAGGAGACGAAGAGAAATCTCGAGACCGGACTAATAA
ACCTTATTCTCTTGGTCCCAAGATCTCTGATTGGGATGAGCAGAGACGTGATTGGCTCAA
ACAAAACCCTAGCTTCCCTAATTTCGTGGCGCCAAACAAGCCTAGGGTTCTTCTTGTCAC
AGGTTCAGCTCCTAAACCGTGTGAGAATCCTGTAGGAGACCATTACCTCTTGAAATCGAT
TAAGAACAAAATCGATTACTGTAGAATACACGGAATCGAGATCTTCTACAACATGGCGTT
GCTCGATGCTGAGATGGCTGGATTCTGGGCTAAGCTTCCGTTGATTAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGA
CGTGTCTAC

Last Updated on 06.12.2018 16:11