Sequences from confirmation amplicon


Confirmation sequences for line 142B03 and hit At1g73990 are shown.
Line ID 142B03
Locus At1g73990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27825924
Primers used for PCR HM37 - 8474, show primer details
   
Sequence Name 29-K032415-022-142-B03C-8409
GenBank Accession LN499175 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K032415-022-142-B03C-8409
CTTTTCTCACGCTCTTTTCTATATCTGTTATCAAGAAGCACACTCAGCATCTCATAATTT
TCCTCAGATATACTTTTGCGAGAAAGCTGATCTCCAGCACTTTTGTATTTTCCAATCCTT
TGTACTTGAGGTTCAATCCCCACTTTTTCGAAGACACCTACATTTTTGAAAAATAACTAG
TTCACGATAAATGTGCCAATTACTGCTTTGTAAAATTGTAATTATTGAAATCCGATTAAG
GACCTTTGATAACGAGAACAGGGAAGTGAGATATGATCACATTAAATTACAAAAGAGGCC
GAGTAGCGGTTCAACAACAGCTTTCTCAACAAATGAAGGATGGTCCGCACACAGTATGAG
TGCATAAGATTTCATTGAACGATATTTAATGACCACTTTTGAAGTGAGAAATTGAGC
   
Sequence Name 29-K032415-022-142-B03C-HM37
GenBank Accession LN499176 [GenBank]
Sequencing primer gene specific primer, HM37: 5'-GATTGTCTTCTTTGGCTCAATTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K032415-022-142-B03C-HM37
TATCGTTCATGAATCTTATGCACTCATACTGTGTGCGGACCATCCTTCATTTGTTGAGAA
AGCTGTTGTTGAACCGCTACTCGGCCTCTTTTGTAATTTAATGTGATCATATCTCACTTC
CCTGTTCTCGTTATCAAAGGTCCTTAATCGGATTTCAATAATTACAATTTTACAAAGCAG
TAATTGGCACATTTATCGTGAACTAGTTATTTTTCAAAAATGTAGGTGTCTTCGAAAAAG
TGGGGATTGAACCTCAAGTACAAAGGATTGGAAAATACAAAAGTGCTGGAGATCAGCTTT
CTCGCAAAAGTATATCTGAGGAAAATTATGAGATGCTGAGTGTGCTTCTTGATAACAGAT
ATTAGAAAAGAGCGTGAGAAAAGTATATATGGAGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACT

Last Updated on 06.12.2018 16:11