Sequences from confirmation amplicon


Confirmation sequences for line 142B12 and hit At2g01250 are shown.
Line ID 142B12
Locus At2g01250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:133590
Primers used for PCR BK86 - 3144, show primer details
   
Sequence Name 19-K105062-0022-142-B12C-BK86
GenBank Accession LN494756 [GenBank]
Sequencing primer gene specific primer, BK86: 5'-GGTTAAGCTTTCCGTAACCTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K105062-0022-142-B12C-BK86
GGATCTGAAAACAGAAGAGAAAAGTCACACTCTTCAAGACTCTTGTTATCTCATAGCATC
AGATTAAAGGGTTTAGGATGAGAGTGTATATACATACCCGTAAGTGACATAGGGCTCAAC
ACGGCGAAGCATGTTCATCGTAGCCTTGTTGACTTTAAGGAAAACACCATTGAAAATCTG
CATTACACAATGCCAAAATCAATCAGTATACAACACTGATAGAATCATTTCATTTCATAT
ATAGTATCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGT
ACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGAT
AGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACAACAATCCA
   
Sequence Name 03-K105063-0022-142-B12C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K105063-0022-142-B12C-3144
TGATTTTGGCACTGTGGGATGCAGATTTC

Last Updated on 06.12.2018 16:11