Sequences from confirmation amplicon


Confirmation sequences for line 142C06 and hit At3g18080 are shown.
Line ID 142C06
Locus At3g18080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6192195
Primers used for PCR VL12 - 8474, show primer details
   
Sequence Name 15-K024393-022-142-C06C-8409
GenBank Accession LN492796 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K024393-022-142-C06C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATAACATTAAGATTATGAATGCAGA
GGGAAGTGGTAAAGTCAACTGGAAAGGAGTTGCTTATTACAATAGATTGATTGATTACAT
GGTTCAAAAAGGTATCAAACCATAACGTTTCTTATAAAACACTGATTTGATTATAGAATG
AAGTTTAACAAATCTAATTTAATGCTTAGGGATTACACCTTATGCTAATCTGTACCACTA
CGATCTTCCATTGGCTTTGGAAAACAAGTATAAAGGCTTGCTTGGTAGACAAGTAGTGTA
AGCACCAATATGCAAAGCTCTTTTATGCATTCCTCAAAAGTTACAAACTTTGATAATGCA
AATTTTACAGGAAAGATTTTGCGGATTACGCTGAGTTTTGTTACAAGACATTTGGTGATA
GAGTGAAAAATTGGATGACGTTCAACGAGCCGCGTGTGGTAGCTGCTTTAGGTTATGATA
ATGGTATTTTCGCACCGGGTAGATGCTCAAAAGCATTTGGAAATTGCACTGAAGGAAACT
CCGCGACAGAGCCATACATAGTTACTCATCACCTTATCTTGGCCCACGCAGCAGCAGTCC
AAAGATACCGCAAATATTACCAAGTAAACAAATAACTTCTAATACGTTTTCTTGATCTTC
TTGATGTGATATGATGAAATAAACCTATATATATGGCTCTTTTGCCAGTGTACTACTAAT
CTTAATGAAA
   
Sequence Name 16-K024393-022-142-C06C-VL12
GenBank Accession LN493265 [GenBank]
Sequencing primer gene specific primer, VL12: 5'-TGAGCTGCAAGATTATCTGCTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K024393-022-142-C06C-vl12
AAGTAAGCGGCTCATACCAAACGAAATCTAAAAGTATCCCAACCCTTCCCTTTTGTTTCG
CCTGGAAAATTTTCATTAAGATTAGTAGTACACTGGCAAAAGAGCCATATATATAGGTTT
ATTTCATCATATCACATCAAGAAGATCAAGAAAACGTATTAGAAGTTATTTGTTTACTTG
GTAATATTTGCGGTATCTTTGGACTGCTGCTGCGTGGGCCAAGATAAGGTGATGAGTAAC
TATGTATGGCTCTGTCGCGGAGTTTCCTTCAGTGCAATTTCCAAATGCTTTTGAGCATCT
ACCCGGTGCGAAAATACCATTATCATAACCTAAAGCAGCTACCACACGCGGCTCGTTGAA
CGTCATCCAATTTTTCACTCTATCACCAAATGTCTTGTAACAAAACTCAGCGTAATCCGC
AAAATCTTTCCTGTAAAATTTGCATTATCAAAGTTTGTAACTTTTGAGGAATGCATAAAA
GAGCTTTGCATATTGGTGCTTACACTACTTGTCTACCAAGCAAGCCTTTATACTTGTTTT
CCAAAGCCAATGGAAGATCGTAGTGGTACAGATTAGCATAAGGTGTAATCCCTAAGCATT
AAATTAGATTTGTTAAACTTCATTCTATAATCAAATCAGTGTTTTATAAGAAACGTTATG
GTTTGATACCTTTTTGAACCATGTAATCAATCAATCTATTGTAATAAGCAACTCCTTTCC
AGTTGACTTTACCACTTCCCTCTGCATTCATAATCTTAATGTTATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA

Last Updated on 06.12.2018 16:11