Sequences from confirmation amplicon


Confirmation sequences for line 142C11 and hit At2g32460 are shown.
Line ID 142C11
Locus At2g32460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13782663
Primers used for PCR DR70 - 8474, show primer details
   
Sequence Name 09-K035534-022-142-C11C-8409
GenBank Accession LN490084 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K035534-022-142-C11C-8409
GCCTTTAAATTGAATATATCCATGTATGATGAATTATTGACGAGCCTTCTCAACAACTTC
CCTTCAACCACAACACCTTTGCCTGATTGGTATCGGGTGACAGAAATGCAAAACGAGGCC
TCATATCTTGCCCCACCAAGTGGAATTCTTATGGGAAACCATCAAGGTAACGGCAGGGTG
GAACCACCCACGGTGCCGCCTTCGTCCAGTGTAGATCCTATGGCCTCGTTGGGGTCATGC
TATTGGAGCAACATGCCTAGCATCTGTTAGTTTGGAACCTGCCCATGAGCAAAAATCATA
TTTCTTTGATTTGAAAAAAAGAGAAAGCTAATTGTGAGCTTAACTTGTGTTTAATGTGTA
AAATCTTCTTCAAAGTGTTTTCAACTTGGGATGTGCTATGAACTGTATGTTAACATGTTT
TCAAGGAACAAAAGAGAAATTGAAAGAGATGAGGAATGAGCATATAATTTTCTTTTGGGT
TAAAGTCATGG
   
Sequence Name 57-K035534-022-142-C11C-DR70
GenBank Accession LN509343 [GenBank]
Sequencing primer gene specific primer, DR70: 5'-CCATGACTTTAACCCAAAAGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K035534-022-142-C11C-DR70
TTCTCTTTTGTTCCTTGAAAACATGTTAACATACAGTTCATAGCACATCCCAAGTTGAAA
ACACTTTGAAGAAGATTTTACACATTAAACACAAGTTAAGCTCACAATTAGCTTTCTCTT
TTTTTCAAATCAAAGAAATATGATTTTTGCTCATGGGCAGGTTCCAAACTAACAGATGCT
AGGCATGTTGCTCCAATAGCATGACCCCAACGAGGCCATAGGATCTACACTGGACGAAGG
CGGCACCGTGGGTGGTTCCACCCTGCCGTTACCTTGATGGTTTCCCATAAGAATTCCACT
TGGTGGGGCAAGATATGAGGCCTCGTTTTGCATTTCTGTCACCCGATACCAATCAGGCAA
AGGTGTTGTGGTTGAAGGGAAGTTGTTGAGAAGGCTCGTCAATAATTCATCATACATGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11