Sequences from confirmation amplicon


Confirmation sequences for line 142D03 and hit At1g22230 are shown.
Line ID 142D03
Locus At1g22230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7851069
Primers used for PCR 08us - 8474, show primer details
   
Sequence Name 39-K117387-0022-142-D03C-08us
GenBank Accession LN503498 [GenBank]
Sequencing primer gene specific primer, 08us: 5'-ATCAGTTGATTCTGTGTCAACGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K117387-0022-142-D03C-08us
TCTTAGTAATCAGATTTTGTATTTGGGTCAATATATANCTCGGGCTTGAAGCATATCATG
TGCATGTCTTTTCCACTTACTGTTTGTATAATTGGCCCATTAAGCAATCGTATTTGTTTG
TTACACGAAAACCAATCAGTCTCAAATTTTCACAATAGTATTATTATAAGAAAGATACTT
AAAATTCACATGTAAATGGCTATATTGGCAATAAATGGGGAATTATATTGTAAATGTAAT
TACCAATATAATTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGC
   
Sequence Name 12-K117386-0022-142-D03C-8409
GenBank Accession LN491766 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117386-0022-142-D03C-8409
TACATTGGTAATTATATTGGTAATTACATTTACAATATAATTCCCCATTTATTGCCAATA
TAGCCATTTACATGTGAATTTTAAGTATCTTTCTTATAATAATACTATTGTGAAAATTTG
AGACTGATTGGTTTTCGTGTAACAAACAAATACGATTGCTTAATGGGCCAATTATACAAA
CAGTAAGTGGAAAAGACATGCACATGATATGCTTCAAGCCCGAGTATATATTGACCCAAA
TACAAAATCTGATTACTTAAGAACAAGACTACTAATCGACGCAAGTATAAGCTTTAGTGC
TTGACGTTGACACAAATCCAACTGAT

Last Updated on 06.12.2018 16:11