Sequences from confirmation amplicon


Confirmation sequences for line 142F02 and hit At2g22660 are shown.
Line ID 142F02
Locus At2g22660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9628013
Primers used for PCR MO03 - 8474, show primer details
   
Sequence Name 07-K024389-022-142-F02C-8409
GenBank Accession LN489092 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K024389-022-142-F02C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAACAAGCAAAGGAAGCC
AACAAGCAAAGGAAGCCAACAAGCATTGTACCTGAACATCACCAAAATCATACAATCACT
TAGAAATATTCACTGATCTAAAAGCAAACACAGAATCTACTTATATTATTAACCTGTAGA
TGGCTTTTTCAAGCGCAGGACCATCGTAGAGCCATCGATTCCTATCAACAGTTTCAAGGA
ACAAAAGGTGTTGTTTCGCTGCAGCTAATAAGTCAACGCTTATTTCTATCTTCTGAGCTT
CAAGCCACTCCACTTCATGATCCTTCTCCTTATCCATATCACCTTTCAAAACGATCAAAA
AATGCAAACAACTAAGTAAATCAATACGTGATTACCAGATTTTGACCTAAAATGATCCTT
ATCTAATCAATTGCATAAGCCCTATGAATTTTGGAAGATCATGTGCATGAGAAAAATCAG
GACTCTGGAGAATCTTAAAA
   
Sequence Name 08-K024389-022-142-F02C-MO03
GenBank Accession LN489556 [GenBank]
Sequencing primer gene specific primer, MO03: 5'-AAAAATCGGGTTTTAAGATTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K024389-022-142-F02C-mo3
CCTGATTTTTCTCATGCACATGATCTTCCAAAATTCATAGGGCTTATGCAATTGATTAGA
TAAGGATCATTTTAGGTCAAAATCTGGTAATCACGTATTGATTTACTTAGTTGTTTGCAT
TTTTTGATCGTTTTGAAAGGTGATATGGATAAGGAGAAGGATCATGAAGTGGAGTGGCTT
GAAGCTCAAAAGATAGAAATAAGCGTTGACTTATTAGCTGCAGCGAAACAACACCTTTTG
TTCCTTGAAACTGTTGATAGGAATCGATGGCTCTACGATGGTCCTGCGCTTGAAAAAGCC
ATCTACAGGTTAATAATATAAGTAGATTCTGTGTTTGCTTTTAGATCAGTGAATATTTCT
AAGTGATTGTATGATTTTGGTGATGTTCAGGTACAATGCTTGTTGGCTTCCTTTGCTTGT
TGGCTTCCTTTGCTTGTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATNTCGACGTGTCTA
Primers used for PCR MO09 - 8474, show primer details
   
Sequence Name 21-K024393-022-142-F02C-8409
GenBank Accession LN495500 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K024393-022-142-F02C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAACAAGCAAAGGAAG
CCAACAAGCAAAGGAAGCCAACAAGCATTGTACCTGAACATCACCAAAATCATACAATCA
CTTAGAAATATTCACTGATCTAAAAGCAAACACAGAATCTACTTATATTATTAACCTGTA
GATGGCTTTTTCAAGCGCAGGACCATCGTAGAGCCATCGATTCCTATCAACAGTTTCAAG
GAACAAAAGGTGTTGTTTCGCTGCAGCTAATAAGTCAACGCTTATTTCTATCTTCTGAGC
TTCAAGCCACTCCACTTCATGATCCTTCTCCTTATCCATATCACCTTTCAAAACGATCAA
AAAATGCAAACAACTAAGTAAATCAATACGTGATTACCAGATTTTGACCTAAAATGATCC
TTATCTAATCAATTGCATAAGCCCTATGAATTTTGGAAGATCATGTGCATGAGAAAAATC
AGGACTCTGGAGAATCTTAAA
   
Sequence Name 22-K024393-022-142-F02C-MO09
GenBank Accession LN495947 [GenBank]
Sequencing primer gene specific primer, MO09: 5'-AAAAATCGGGTTTTAAGATTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K024393-022-142-F02C-mo9
TCCTGATTTTTCTCATGCACATGATCTTCCAAAATTCATAGGGCTTATGCAATTGATTAG
ATAAGGATCATTTTAGGTCAAAATCTGGTAATCACGTATTGATTTACTTAGTTGTTTGCA
TTTTTTGATCGTTTTGAAAGGTGATATGGATAAGGAGAAGGATCATGAAGTGGAGTGGCT
TGAAGCTCAGAAGATAGAAATAAGCGTTGACTTATTAGCTGCAGCGAAACAACACCTTTT
GTTCCTTGAAACTGTTGATAGGAATCGATGGCTCTACGATGGTCCTGCGCTTGAAAAAGC
CATCTACAGGTTAATAATATAAGTAGATTCTGTGTTTGCTTTTAGATCAGTGAATATTTC
TAAGTGATTGTATGATTTTGGTGATGTTCAGGTACAATGCTTGTTGGCTTCCTTTGCTTG
TTGGCTTCCTTTGCTTGTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11