Sequences from confirmation amplicon


Confirmation sequences for line 142H11 and hit At1g65930 are shown.
Line ID 142H11
Locus At1g65930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24541417
Primers used for PCR ms14 - 8474, show primer details
   
Sequence Name 01-K024389-022-142-H11C-8409
GenBank Accession LN486415 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K024389-022-142-H11C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTCAATCGTCACTTCAG
GGTTCATCAGAAAGGTGGTGAGACCAGCACAAACAGCATAGCCTCTATTTTTGCTTGGAC
TCGTGGACTTGCACACAGGTAACAAAAAGAAAACATGCTTAATAATGTTTCATTAGAAGG
AAGAGGATTGTCTAAGCTAATGTCTTTGAAACAGGGCTAAGTTAGATGACAACGCAAAAC
TCTTGGATTTCACTGAGAAGCTGGAAGCCGCTTGTGTTGGGACAGTGGAGTCAGGGAAAA
TGACCAAAGATCTTGCACTCATCATTCACGGCTCAAAGTAATCAATTCAACACTTTCTAT
TTACGTCCTTGTTCTCATCAAGATCACTTAATTTACTGTCGGATTTTTTCAGGCTTTCGA
GGGACACGTACCTGAACACAGAGGAGTTCATCGATGCAGTCGCTGCTGAGCTCAAAGAAA
GACTCAATGCTTAAGAAACTGAAGAAATTGGGTAAAATTCGAACGTC
   
Sequence Name 02-K024389-022-142-H11C-ms14
GenBank Accession LN486871 [GenBank]
Sequencing primer gene specific primer, ms14: 5'-TAGGAACTGAGACGTTCGAATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K024389-022-142-H11C-ms14
CCAATTTCTTCAGTTTCTTAAGCATTGAGTCTTTCTTTGAGCTCAGCAGCGACTGCATCG
ATGAACTCCTCTGTGTTCAGGTACGTGTCCCTCGAAAGCCTGAAAAAATCCGACAGTAAA
TTAAGTGATCTTGATGAGAACAAGGACGTAAATAGAAAGTGTTGAATTGATTACTTTGAG
CCGTGAATGATGAGTGCAAGATCTTTGGTCATTTTCCCTGACTCCACTGTCCCAACACAA
GCGGCTTCCAGCTTCTCAGTGAAATCCAAGAGTTTTGCGTTGTCATCTAACTTAGCCCTG
TTTCAAAGACATTAGCTTAGACAATCCTCTTCCTTCTAATGAAACATTATTAAGCATGTT
TTCTTTTTGTTACCTGTGTGCAAGTCCACGAGTCCAAGCAAAAATAGAGGCTATGCTGTT
TGTGCTGGTCTCACCACCTTTCTGATGAACCCTGAAGTGACGATTGAATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACAT
TCACCCCC

Last Updated on 06.12.2018 16:11