Sequences from confirmation amplicon


Confirmation sequences for line 143A02 and hit At1g25580 are shown.
Line ID 143A02
Locus At1g25580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8999673
Primers used for PCR AB43 - 8474, show primer details
   
Sequence Name 40-K104096-0022-143-A02C-AB43
GenBank Accession LN503688 [GenBank]
Sequencing primer gene specific primer, AB43: 5'-AGCACATTTCTGGGAGAAAAAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K104096-0022-143-A02C-AB43
CCCAACCTAGCAGTGAAAGACCCAAATGCAGATTTTCAAATTAACCCCTTTCGCGCTTAA
CTTTCACATCGGAGAAATTGAAATCACAAAGCTCAATTTTGATATATAAACGCAAACAAA
AAAAGATTGAAACTTTAGTTTGTACTTCGGAATTGAAATCGGCAGGAATCGTACTTAGGG
TTTAGAGCCTCAACTAAAAACTCCATGACAGAGTCAAAATCCAGAACATGATAGAAGAAA
TAAAGTACTTACCCAGCCATTAGAGCTCGATGAAGAAAAGATCAGAGCAAAGGAGAAGCA
GAATCTGAGAATAAACCCTAGACAAGTCTCTCTAGAAGCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 56-K104097-0022-143-A02C-8409
GenBank Accession LN509084 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K104097-0022-143-A02C-8409
AAGCCATTTACAATTGAATATATCCTGCTTCTAGAGAGACTTGTCTAGGGTTTATTCTCA
GATTCTGCTTCTCCTTTGCTCTGATCTTTTCTTCATCGAGCTCTAATGGCTGGGTAAGTA
CTTTATTTCTTCTATCATGTTCTGGATTTTGACTCTGTCATGGAGTTTTTAGTTGAGGCT
CTAAACCCTAAGTACGATTCCTGCCGATTTCAATTCCGAAGTACAAACTAAAGTTTCAAT
CTTTTTTTGTTTGCGTTTATATATCAAAATTGAGCTTTGTGATTTCAATTTCTCCGATGT
GAAAGTTAAGCGCGAAAGGGGTTAATTTGAAAATCTGCATTTGGGTCTTTCACTGCTAGG
TTGGGGTTTTTGAAGTTTCCAGTCCTATGATACTTTTTCTCCCAANAATGTGC

Last Updated on 06.12.2018 16:11