Sequences from confirmation amplicon


Confirmation sequences for line 143C01 and hit At4g32800 are shown.
Line ID 143C01
Locus At4g32800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15820697
Primers used for PCR CM79 - 8474, show primer details
   
Sequence Name 13-K104151-0022-143-C01C-CM79
GenBank Accession LN492016 [GenBank]
Sequencing primer gene specific primer, CM79: 5'-ATATTTGACTCGTTGGAGGATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K104151-0022-143-C01C-CM79
CGGTCGGAAGAGAGTTTTTCGTATAATAACAATGATTCTTCGTTGAATTACTCATCAGTT
TTCGAATCTTGGAAACATTTTCCCTGAGAAAAGAAAGAAGCCATATTTTCTCTCTCTTTT
TCGCCATTTTTGGTTTTAAGTTTTTTGATGGGATTCTCTCTGTTTTTGCTATGCGTGAGA
TATCTCTCTTTGTGGAATAGATAACTTTTCTGTTGAAAATCTCCTAAGTCTGTGAAATGA
ACGACGATGGAAATGTAAGTGTGTATATTAGCCCGAAATAATGACTGTATACATTCTTTT
TTGCAAGTGTGTATATTTATTTTTTTCGGGCTAATATACAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 13-K104152-0022-143-C01C-8409
GenBank Accession LN492017 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K104152-0022-143-C01C-8409
CGGAATGAGCCATTTACATTGAATAATCCTGTATATTAGCCCGAAAAAAATAAATATACA
CACTTGCAAAAAAGAATGTATACAGTCATTATTTCGGGCTAATATACACACTTACATTTC
CATCGTCGTTCATTTCACAGACTTAGGAGATTTTCAACAGAAAAGTTATCTATTCCACAA
AGAGAGATATCTCACGCATAGCAAAAACAGAGAGAATCCCATCAAAAAACTTAAAACCAA
AAATGGCGAAAAAGAGAGAGAAAATATGGCTTCTTTCTTTTCTCAGGGAAAATGTTTCCA
AGATTCGAAAACTGATGAGTAATTCAACGAAGAATCATTGTTATTATACGTAAAAACTTC
TTCTTCCGTACCGCTTAACCACCGAGGCATGTACACCAAATCCTCCAACAGNTCAAAT

Last Updated on 06.12.2018 16:11