Sequences from confirmation amplicon


Confirmation sequences for line 143C03 and hit At5g40090 are shown.
Line ID 143C03
Locus At5g40090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16043204
Primers used for PCR BW14 - 8474, show primer details
   
Sequence Name 73-K104358-0022-143-C03C-BW14
GenBank Accession LN512890 [GenBank]
Sequencing primer gene specific primer, BW14: 5'-CTGACTTCGTACACATCCATCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K104358-0022-143-C03C-BW14
ATGACTCTGCTTCCTGGAGCAACCAGTTAGCATATTCAATGATCCACTTCCCTTGTTCCA
TGTTGTTCACATCATCAGCGATAAGGAGAATTTTCCGGTGTTTACGCCTTGCTTCGGTAA
TTTCATGTCCATCGTGATATGAGCTTATTATTACCGTTGGATCTTCTTCTCCTTCAAACT
TTAGAAGCTTGTCTTTCATGTTTTCAACATTATCTAGGAAAACATGTGTCTGAAAGTTTA
CAAAGATCTCTGCATAAATGTACCTTGCAAGTGTTGTCTTCCCAACACCAGCACTACCCC
AAATGCCAATTGTACGAACCTCTTTGTTAACTTCTAAAGCCAACAAGTCATAAACCACTT
TCATGTGACGATCCATTGCAACAAGGGCATTCGAATCGTTTGATCCGGAGTAGAACACGA
CAAGAGATATGTCACGAGCAATTTGGTTGAGTAGATCTGACTGATCCATTCCTGCAAGAT
TTTGAGATAATGGATACTCAGCGGCAAGGTTGGTCAATTTGGTAAGTGCAGTTCTCCAAC
TTGGCGCCTTCTCCGGATGTTGTCTGCATATTTCTTCCACATTAAAAGATAAATCTGTAA
GAAAAATGGGTATGACCATGAGTGAACCTTTTTCCTGATTCAATTGTAAATGGCTTCATG
TCCNGGGAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGA
GTAATTACCAATTTTTTTTCAATTTCAAAATGT
   
Sequence Name 81-K104359-0022-143-C03C-8409
GenBank Accession LN514064 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K104359-0022-143-C03C-8409
CATTTCATTGAATCGGAAAAAGGTTCACTCATGGTCATACCCATTTTTCTTACAGATTTA
TCTTTTAATGTGGAAGAAATATGCAGACAACATCCGGAGAAGGCGCCAAGTTGGAGAACT
GCACTTACCAAATTGACCAACCTTGCCGCTGAGTATCCATTATCTCAAAATCTTGCAGGA
ATGGATCAGTCAGATCTACTCAACCAAATTGCTCGTGACATATCTCTTGTCGTGTTCTAC
TCCGGATCAAACGATTCGAATGCCCTTGTTGCAATGGATCGTCACATGAAAGTGGTTTAT
GACTTGTTGGCTTTAGAAGTTAACAAAGAGGTTCGTACAATTGGCATTTGGGGTAGTGCT
GGTGTTGGGAAGACAACACTTGCAAGGTACATTTATGCAGAGATCTTTGTAAACTTTCAG
ACACATGTTTTCCTAGATAATGTTGAAAACATGAAAGACAAGCTTCTAAAGTTTGAAGGA
GAAGAAGATCCAACGGTAATAATAAGCTCATATCACGATGGACATGAAATTACCGAAGCA
AGGCGTAAACACCGGAAAATTCTCCTTATCGCTGATGATGTGAACAACATGGAACAAGGG
AAGTGGATCATTGAATATGCTAACTGGTTTGCTCCAGGAAGCAGAGTCATTCTTATTAGC
CAGAACAAGAATTTGCTTGTTGACGCCGGAGTGATGGATGT

Last Updated on 06.12.2018 16:11