Sequences from confirmation amplicon


Confirmation sequences for line 143C06 and hit At5g62420 are shown.
Line ID 143C06
Locus At5g62420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25065857
Primers used for PCR BU58 - 8474, show primer details
   
Sequence Name 12-K104400-0022-143-C06C-BU58
GenBank Accession LN491562 [GenBank]
Sequencing primer gene specific primer, BU58: 5'-ACCAAAGGAAGATGAGTTTGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K104400-0022-143-C06C-BU58
GCAGGCATGGAGAGATGCTTGGAGATGGGTTTGTGCAGATCTATTGGTGTTAGCAACTTC
TCTTCTAAGAAGATCTTTGATCTCCTTGATTTCGCTTCTGTCTCTCCATCGGTGAATCAG
GTTTGGATTCAACATTACACCCAAGATTTCTTGTTGGAGAAATTCAAGAAACTAAATATT
TTCATATATGCATGTATAGGTGGAAATGCATCCATTGTGGAGACAAAGAAAGCTAAGGAA
AGTGTGTGAAGAGAACAATATCCATGTAAGTGGATACTCCCCACTTGGTGGGCCAGGGAA
TTGTTGGGGATCAACGGCTGTGATTGAACATCCTATCATCAAATCCATTGCTCTCAAGCA
TAATGCCACTCCTGCTCAGGTAACTCCACAATTTAAGGTCCTTCCTCTTTTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 28-K104401-0022-143-C06C-8409
GenBank Accession LN498829 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104401-0022-143-C06C-8409
CATTTACAAAAGAGGAAGGACCTTAAATTGTGGAGTTACCTGAGCAGGAGTGGCATTATG
CTTGAGAGCAATGGATTTGATGATAGGATGTTCAATCACAGCCGTTGATCCCCAACAATT
CCCTGGCCCACCAAGTGGGGAGTATCCACTTACATGGATATTGTTCTCTTCACACACTTT
CCTTAGCTTTCTTTGTCTCCACAATGGATGCATTTCCACCTATACATGCATATATGAAAA
TATTTAGTTTCTTGAATTTCTCCAACAAGAAATCTTGGGTGTAATGTTGAATCCAAACCT
GATTCACCGATGGAGAGACAGAAGCGAAATCAAGGAGATCAAAGATCTTCTTAGAAGAGA
AGTTGCTAACACCAATAGATCTGCACAAACCCATCTCCAAGCATCTCTCCATGCCTTGCC
AAGTTTCTTCAATTCCCAAATCTTTCTCAAACT
Primers used for PCR 19ah - 3144, show primer details
   
Sequence Name 06-K106400-0022-143-C06C-3144
GenBank Accession LN488824 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K106400-0022-143-C06C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTTAT
TCATTGACTTATGGCTATTAATGAAGGTGGCTCTAAGATGGGGAATGTCAAAAGGAGCAA
GTGTGATCGTCAAGAGTTTTAATGGAGCAAGAATGATAGAGAACAAGAGAGCCTTAGAGA
TTAAATTGGATGATCAAGACTTGTCACTCATTGACCATTTGGAGGAATGGAAGATCATGA
GAGGAGACTTCCTTGTTAATCAAACCACAAGCCCTTACAAATCCATCCAACAACTTTGGG
ATAATGAGATTTAATTTATTAGGGGTTCACATCATTCACTTCAAAATGTAATTTTATCTT
CTCATTCTCTTTGCTTATGATCTTTTTCACATTC
   
Sequence Name 22-K106401-0022-143-C06C-19ah
GenBank Accession LN496126 [GenBank]
Sequencing primer gene specific primer, 19ah: 5'-CGAATGTGAAAAAGATCATAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K106401-0022-143-C06C-19ah
ATTACATTTTGAGTGATGATGTGACCCCTAATAAATTAAATCTCATTATCCCAAAGTTGT
TGGATGGATTTGTAAGGGCTTGTGGTTTGATTAACAAGGAAGTCTCCTCTCATGATCTTC
CATTCCTCCAAATGGTCAATGAGTGACAAGTCTTGATCATCCAATTTAATCTCTAAGGCT
CTCTTGTTCTCTATCATTCTTGCTCCATTAAAACTCTTGACGATCACACTTGCTCCTTTT
GACATTCCCCATCTTAGAGCCACCTTCATTAATAGCCATAAGTCAATGAATAAAACACTG
ATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAA
ATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC
ATCCCTTACGTCAGTGGAGATATCACAACAATCCA

Last Updated on 06.12.2018 16:11