Sequences from confirmation amplicon


Confirmation sequences for line 143C11 and hit At1g54790 are shown.
Line ID 143C11
Locus At1g54790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20442976
Primers used for PCR 09qz - 8474, show primer details
   
Sequence Name 28-K115914-0022-143-C11C-09qz
GenBank Accession LN498990 [GenBank]
Sequencing primer gene specific primer, 09qz: 5'-AATCCCCAAAACATTAACATGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K115914-0022-143-C11C-09qz
ATATACTCTCTTAAATTGAAGCGCAAGGACACTAACGGGTTTTCTTCGTTCGGAACCAAA
CTAATCATCCCTTTAAAGAAAAAAATAATCTCGATAAATATTCCTCACGAAGCAACCTCG
GAGCATTCTCGAAGCAACATTATTTCATACATTTCTGAGTCTGATATATCTCCGTTCAAT
TAAAAATTCATGTTCTAAATATAACAAAATGCGGTTTTAATAGTGCAAACAATTACAAAT
ATGTGATTGTAATTGAGAAAATAATTATTAATCCGATTACTAAGTTTGTATATTTTTGTA
TGTTTCTATTAAATGAATTCTTAGAATTGTAGAATTACCAATGGATCAGCAATGAATAAT
TGAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGC
   
Sequence Name 02-K115913-0022-143-C11C-8409
GenBank Accession LN487177 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115913-0022-143-C11C-8409
TATTCAATTATTCATTGCTGATCCATTGGTAATTCTACAATTCTAAGAATTCATTTAATA
GAAACATACAAAAATATACAAACTTAGTAATCGGATTAATAATTATTTTCTCAATTACAA
TCACATATTTGTAATTGTTTGCACTATTAAAACCGCATTTTGTTATATTTAGAACATGAA
TTTTTAATTGAACGGAGATATATCAGACTCAGAAATGTATGAAATAATGTTGCTTCGAGA
ATGCTCCGAGGTTGCTTCGTGAGGAATATTTATCGAGATTATTTTTTTCTTTAAAGGGAT
GATTAGTTTGGTTCCGAACGAAGAAAACCCGTTAGTGTCCTTGCGCTTTCAATTTTAATG
AGAGTTATGATTTTTTTTATTTTTTTTTCATGTTAAGTTGTGGGGGGATTA

Last Updated on 06.12.2018 16:11