Sequences from confirmation amplicon


Confirmation sequences for line 143D01 and hit At3g21055 are shown.
Line ID 143D01
Locus At3g21055 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7376810
Primers used for PCR KP33 - 8474, show primer details
   
Sequence Name 47-K030459-022-143-D01C-8409
GenBank Accession LN506095 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K030459-022-143-D01C-8409
ACGCACAAGTTTGTGTTCACAATGCCTACGGCCAAGATCTGCCGCTACTGATTCAACAAC
CCACCTTCTGCACGTCTATCTCTCTCTTTCAATTTCGGTAAATTTTCTCAATTAAGTTTG
GTCTGTAATCGTATATTTGATCATCAATTTACCAAAAGCCCTTTTATCTTTCTACAAGAA
TCGTCTTCTTTCACCATTAGCAAAACTTGACAAAAGCATTGCATTGTGGATACAAAACAG
TTTCCCAAAAACTAGATTGATTCCAAACCGTCCTAGGTTAAAAAAAGACCACACTAATCC
ATACTGCAGCAGCAGTAGCTTTCTTATTCTTAGGCAAAGAAACTTAAAACAGAAGAATAC
CAAACTAATACGTCCACCATACGAGTTAAGCCTAGTGCCAGATAACTTCTTTAATCACCT
GCAAACGAATAACATAACCTGTCTCTTCCAACATGGCTTCTCCAAATACTTTCCCAGTGT
GATTCTTCATAATGCATTTGCCTCCTCCAAATGTTACTGA
   
Sequence Name 48-K030459-022-143-D01C-KP33
GenBank Accession LN506408 [GenBank]
Sequencing primer gene specific primer, KP33: 5'-AGACCAAGGCTGTTCAGTAACATT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K030459-022-143-D01C-KP33
AAGAAGTTATCTGGCACTAGGCTTAACTCGTATGGTGGACGTATTAGTTCGGTATTCTTC
TGTTTTAAGTTTCTTCGCCTAAGAATAAGAAAGCTACTGCTGCTGCAGTATGGATTAGTG
TGGTCTTTTTTTAACCTAGGACGGTTTGGAATCAATCTAGTTTTTGGGAAACTGTTTTGT
ATCCACAATGCAATGCTTTTGTCAAGTTTTGCTAATGGTGAAAGAAGACGATTCTTGTAG
AAAGATAAAAGGGCTTTTGGTAAATTGATGATCAAATATACGATTACAGACCAAACTTAA
TTGAGAAAATTTACCGAAATTGAAAGAGAGAGATAGACATGCAGAAGGTGGGTTGTTGAA
TCAGTAGCGGCAGATCTTGGCCGTAGGCATTGTGACACAAACTTGTGCGTAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CT

Last Updated on 06.12.2018 16:11