Sequences from confirmation amplicon


Confirmation sequences for line 143D02 and hit At1g16120 are shown.
Line ID 143D02
Locus At1g16120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5523226
Primers used for PCR 30mk - 3144, show primer details
   
Sequence Name 73-K114351-0022-143-D02C-30mk
GenBank Accession LN512948 [GenBank]
Sequencing primer gene specific primer, 30mk: 5'-CCTTCGCATGAATCAGTATCTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K114351-0022-143-D02C-30mk
AAGAAGAAAACATCACTTATGACTTACCTACGCAGCCACCTCGAAGGTATGGGTTCCCTG
TGTAGCCATAGTCGCAATAACAATTCCTATAACTCATCCCGGAGAAGTAATCATACTCGC
AACTGCAATTATCATCCGAGAACCCAGAACCTTTTCGGCTCATATTTGTACAACCCAAGA
GGCTCTTAAAGCGAGAGTTCGAAGTGGCAAAGTACCATCCTAACTCTAGCACCACATATC
CATTGTTATGAAACTGTTCTGGCTCTGTAACATTAGACGGCGAATACCTCTTGTCCGTCA
AGAACGCAACTTTGCACGTTTCTTCTCCTCCACTAGAGTTCTCTATATTTACGGTTATAG
CCTGAGGACGCCCCACCGGTAGCATCTGTAAATTATTACCGTCTTTATCAAGTTACCCGG
GTAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGT
CCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGA
TTGTGCGTCATCCCTTACGTCAGTGGA
   
Sequence Name 25-K114350-0022-143-D02C-3144
GenBank Accession LN497551 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K114350-0022-143-D02C-3144
CCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTACCCGGGTAACTT
GATAAAGACGGTAATAATTTACAGATGCTACCGGTGGGGCGTCCTCAGGCTATAACCGTA
AATATAGAGAACTCTAGTGGAGGAGAAGAAACGTGCAAAGTTGCGTTCTTGACGGACAAG
AGGTATTCGCCGTCTAATGTTACAGAGCCAGAACAGTTTCATAACAATGGATATGTGGTG
CTAGAGTTAGGATGGTACTTTGCCACTTCGAACTCTCGCTTTAAGAGCCTCTTGGGTTGT
ACAAATATGAGCCGAAAAGGTTCTGGGTTCTCGGATGATAATTGCAGTTGCGAGTATGAT
TACTTCTCCGGGATGAGTTATAGGAATTGTTATTGCGACTATGGCTACACAGGGAACCCA
TACCTTCGAGGTGGCTGCGTAGGTNAGTCATAAGTGATTGTTTTCTTCTTTTGTTTTCTC
ATTAGATCATCAATAATTATGTTTCAAAATTGTGTAGATACT

Last Updated on 06.12.2018 16:11