Sequences from confirmation amplicon


Confirmation sequences for line 144E03 and hit At3g62110 are shown.
Line ID 144E03
Locus At3g62110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22998629
Primers used for PCR KB62 - 3144, show primer details
   
Sequence Name 06-K100818-0022-144-E03C-3144
GenBank Accession LN488724 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K100818-0022-144-E03C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTAACAA
GTTGTATAAGTGATCACAGTTCATATAGCAAAGGGTATGCTTGTAAAGATTCAAAAAGGC
AGAGACTTGCCGTTGACCCGAGAATCGTTGCACCTTTATCTAGCCATAAGGTGAGGTGAC
TGATAAGGTCGAAACTCCCGGTAAGCCACTGACCAGCGGGAACAAAAAGCTTGGCCCCGC
CTTTATCAGAGAAAGAATTGAGGTAGAACAGAGCATTCTGAAAGGCTTTAGTGTTTAGAG
TCACACCATCTCC
   
Sequence Name 06-K100830-0022-144-E03C-KB62
GenBank Accession LN488726 [GenBank]
Sequencing primer gene specific primer, KB62: 5'-TGGAGATGGTGTGACTCTAAACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K100830-0022-144-E03C-KB62
TCATTCTTTCTCTGATAAGGCGGGGCCAAGCTTTTTGTTCCCGCTGGTCAGTGGCTTACC
GGGAGTTTCGACCTTATCAGTCACCTCACCTTATGGCTAGATAAAGGTGCAACGATTCTC
GGGTCAACGGCAAGTCTCTGCCTTTTTGAATCTTTACAAGCATACCCTTTGCTATATGAA
CTGTGATCACTTATACAACTTGTTAACACTGATAGTTTAAACCGAAGGCGGGAAACGACA
ATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAG
GGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGG
Primers used for PCR KB61 - 8474, show primer details
   
Sequence Name 45-K100820-0022-144-E03C-8409
GenBank Accession LN505452 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K100820-0022-144-E03C-8409
CATTTACAATTGAATATATCCGTGGTTGATCCATTACCATCATATGGACGAGGAAGAGAA
TTGCCCGGTAGAAGACACAGAAGTCTTATATATGGTCAGAATCTCACAGATGTAGTCATA
ACAGGTACTAGTACAGCATTTATAAAAAATGATTAGTTTTGAACTGATGATGCTAGTTTT
AGATATGCTGTCTGATGATATCAACATTTTAGGCGAGAACGGGACAATTGATGGTCAAGG
AACTGTATGGTGGGATTGGTTTAGAAATGGAGAGCTTAACTATACAAGACCTCATCTTGT
TGAGCTCATGAACTCTACTGGTCTGATCATCTCCAACCTCACATTCTTGAATTCACCGTT
TTGGAACATTCATCCTGTTTATTGCAGGTCATAATAACTTAATCATGATTTGATTTTGAT
ATCCACCAAATTTCTCTCTTCAATGTTCTGATATGGAGTTTTTGTCTGAACAGAGATGTT
GTTGTAAAGAATCTCACAATTTTGGCTCCTCTTGAATCTCCAAATACAGATGGAGTTGAT
CCAGGTGAGTTTCCTTGCAAATTTTACTCGTAGATTCAAGATATGTACCTTATTATTATC
TTTGGAAATTGTTCTGTTG
   
Sequence Name 45-K100832-0022-144-E03C-KB61
GenBank Accession LN505454 [GenBank]
Sequencing primer gene specific primer, KB61: 5'-TGAATCTGCAAACAGAACAATTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K100832-0022-144-E03C-KB61
TATCTTGATCTACGAGTAAATTTGCAAGGAAACTCACCTGGATCAACTCCATCTGTATTT
GGAGATTCAAGAGGAGCCAAAATTGTGAGATTCTTTACAACAACATCTCTGTTCAGACAA
AAACTCCATATCAGAACATTGAAGAGAGAAATTTGGTGGATATCAAAATCAAATCATGAT
TAAGTTATTATGACCTGCAATAAACAGGATGAATGTTCCAAAACGGTGAATTCAAGAATG
TGAGGTTGGAGATGATCAGACCAGTAGAGTTCATGAGCTCAACAAGATGAGGTCTTGTAT
AGTTAAGCTCTCCATTTCTAAACCAATCCCACCATACAGTTCCTTGACCATCAATTGTCC
CGTTCTCGCCTAANATGTTGATATCATCAGACAGCATATCTNANACTAGCATCATCAGTT
CAAAACTAATCATTTTTTATAAATGCTGTACTAGTACCTGTTATGACTACNTCTGTGAGA
TTCTGACCATATATNAGACTTCTGTGTCTTCTACCGGGCAATTCTCTTCCTCGTCCATAT
GATGGTAATGGATCAACCACGGATATNTTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCANAAATGTAGATT

Last Updated on 06.12.2018 16:11