Sequences from confirmation amplicon


Confirmation sequences for line 144F03 and hit At3g48080 are shown.
Line ID 144F03
Locus At3g48080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17753031
Primers used for PCR 02uc - 8474, show primer details
   
Sequence Name 34-K117029-0022-144-F03C-02uc
GenBank Accession LN501568 [GenBank]
Sequencing primer gene specific primer, 02uc: 5'-GATGTGTTTTGGAGCAAGGTAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117029-0022-144-F03C-02uc
GGCTGAGGGCGAAGAACTCAAGGGAAAGCCATATGAGGAAGTTCAGGTAAGATTCAAGAC
ACTAGAAGGGTTGCTTGAAGGATGGATCAAAGACGGGGAAGTAGATGAGAAGGAAATATT
TCTGGAGGGTTCAACATTTAGAAAGTGGTGGAATACACTTCCTGACAGTCACAAAATACA
TGCTCCGCTGTATCCACGTGAGCGTATGATGGATGAAACAAGAGCTACCTGAACCTTAAA
TGGTTCGATTATTGAAACACTTGCTTCTTAATTTGTGCAATAAGAAATGTTTATCAATCT
GGTTTCCACTTCACGATGTAAATGGCAATCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCC
   
Sequence Name 02-K117028-0022-144-F03C-8409
GenBank Accession LN487205 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117028-0022-144-F03C-8409
AAACCAGATGATAAACATTTCTTATTGCACAAATTAAGAAGCAAGTGTTTCAATAATCGA
ACCATTTAAGGTTCAGGTAGCTCTTGTTTCATCCATCATACGCTCACGTGGATACAGCGG
AGCATGTATTTTGTGACTGTCAGGAAGTGTATTCCACCACTTTCTAAATGTTGAACCCTC
CAGAAATATTTCCTTCTCATCTACTTCCCCGTCTTTGATCCATCCTTCAAGCAACCCTTC
TAGTGTCTTGAATCTTACCTGAACTTCCTCATATGGCTTTCCCTTGAGTTCTTCGACCTC
AGCCCAAAAGCATGAACCGCACTCGGATCCCGAATTCTTTAAGATCTCTTGAATGTCTTG
CTGTAATCCTAAGTTAAGACCATTTACCTTGCTCAAAAACACATC

Last Updated on 06.12.2018 16:11