Sequences from confirmation amplicon


Confirmation sequences for line 144F09 and hit At3g45670 are shown.
Line ID 144F09
Locus At3g45670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16766246
Primers used for PCR 11pu - 8474, show primer details
   
Sequence Name 26-K115234-0022-144-F09C-11pu
GenBank Accession LN498056 [GenBank]
Sequencing primer gene specific primer, 11pu: 5'-CTTTTCATGTCTAAATTGGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115234-0022-144-F09C-11pu
TCTTTTCTTTTAAGGTTTCTTCGCTATATTGCTTGACGAGGGAATAAAGTATCGCCTACT
ATGGCCGGGTCAGAAGGAGCTAGCAATCTCCATTCCAAGATTCCAGCTATGTACAGACAC
AAAATATGCAAGATCACTGCAAAAAATCTATCTTTTGGTAAAGGAAGGATCTTGGTGAAT
AGGGAGACTCGGTTTTCGGTGAATTGATACAGTGACTACTCTACTTTGCAACTGCAAACT
AATAATGAAACAGAGAACTCAACATGAATCCATTAACAACAATTATAATACTTTACCAAA
TACTACAAAATACTTGCAGTTACCAATACTACGAAACAGAACTCAATCATAAACAAACTC
AATCATAAACAAATCCATTGTGAGAAAACTCTTCATCTGAACAGAGAAACGGATGGTCCA
CGAGTTCACCGATGGTCCACCTCGAACCAGGCTCCTTAACCAAACACCTCATCAGAAAGT
CTTGAGCTTCTGCAGATAACCGAATCGAGATTTGAGGAGTGAGATCACTGTGACCGATCA
AATCAACCCAATCATCATGAGCCAAATCACCAATATGGGCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 02-K115233-0022-144-F09C-8409
GenBank Accession LN487158 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115233-0022-144-F09C-8409
GCATTTCATTGAATATATCCTGCCCATATTGGTGATTTGGCTCATGATGATTGGGTTGAT
TTGATCGGTCACAGTGATCTCACTCCTCAAATCTCGATTCGGTTATCTGCAGAAGCTCAA
GACTTTCTGATGAGGTGTTTGGTTAAGGAGCCTGGTTCGAGGTGGACCATCGGTGAACTC
GTGGACCATCCGTTTCTCTGTTCAGATGAAGAGTTTTCTCACAATGGATTTGTTTATGAT
TGAGTTTGTTTATGATTGAGTTCTGTTTCGTAGTATTGGTAACTGCAAGTATTTTGTAGT
ATTTGGTAAAGTATTATAATTGTTGTTAATGGATTCATGTTGAGTTCTCTGTTTCATTAT
TAGTTTGCAGTTGCAAAGTAGAGTAGTCACTGTATCAATTCACCGAAAACCGAGTCTCCC
TATTCACCAAGATCCTTCCTTTACCAAAAGATAGATTTTTTGCAGTGATCTTGCATATTT
TGTGTCTGTACATAGCTGGAATCTTGGAATGGAGATTGCTAGCTCCTTCTGACCCGGCCA
TAGTAGGCGATACTTTATTCCCTCGTCAAGCAATATAGCTGAAGAAACCTATAAAAGAAA
AGACTGAAACCATCACAAAAGAAAAAGAGCTCGAGAAACCAATTTAGACATGAAAA

Last Updated on 06.12.2018 16:11