Sequences from confirmation amplicon


Confirmation sequences for line 144G10 and hit At3g17770 are shown.
Line ID 144G10
Locus At3g17770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6085664
Primers used for PCR BK88 - 8474, show primer details
   
Sequence Name 09-K105704-0022-144-G10C-BK88
GenBank Accession LN490176 [GenBank]
Sequencing primer gene specific primer, BK88: 5'-GTTTTGATAGCGGCTTAATACTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K105704-0022-144-G10C-BK88
GAATTATCAACAACCCCACGGTTCGTTCTTCTTCGTCTTCCTCCTTCCTTTTTTTTCTTG
CGTTTCTCTGTTATTCGCATCCCCGACATTTTACTTTCTCCGGCGTAGATTAGGTCGATT
AGAATTGTGAACGCTCTGGCGAACTGTTGACCTATGTTAATTTTGTTTCCCGATTGTGTT
ACTGTTTGCGAAATGATTTGCATTGCGAGTCTCTTGCTCATAAAATTCGCCTTGGTCTAT
TTATCCACATTTTCCCCCCAATTTTGTTCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 25-K105705-0022-144-G10C-8409
GenBank Accession LN497443 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K105705-0022-144-G10C-8409
GGAATGAGCCATTTACAATTGAATATATCCTGAACAAAATTGGGGGGAAAATGTGGATAA
ATAGACCAAGGCGAATTTTATGAGCAAGAGACTCGCAATGCAAATCATTTCGCAAACAGT
AACACAATCGGGAAACAAAATTAACATAGGTCAACAGTTCGCCAGAGCGTTCACAATTCT
AATCGACCTAATCTACGCCGGAGAAAGTAAAATGTCGGGGATGCGAATAACAGAGAAACG
CAAGAAAAAAAAGGAAGGAGGAAGACGAAGAAGAACGAACCGTTGGGGTTGTTGATGAAT
TTCTTGGCTGCGAAATCCATAATTGCGAGTATTAAGCCCTTATCAAAA

Last Updated on 06.12.2018 16:11