Sequences from confirmation amplicon


Confirmation sequences for line 145C04 and hit At5g25120 are shown.
Line ID 145C04
Locus At5g25120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8664284
Primers used for PCR MS66 - 8474, show primer details
   
Sequence Name 39-K017401-022-145-C04C-MS66
GenBank Accession LN503181 [GenBank]
Sequencing primer gene specific primer, MS66: 5'-TAGTGGCTTCTATATCGGCAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K017401-022-145-C04C-ms66
AATCGAATTCTGATAATGCCTCTCCTCAATCCAAAAAAAACATAAGATACGTACATCATG
CTAAATGTAAAGACACATAACACTTCAAAAAACCGTTTTACATTACTTCTTTCAGCTATT
TTTTAATGTTCATTTCATATGGTAGCATTGTAGTTAACTTTCTCAACATAAGTCTTTAAT
AATCTAATGTCTACACATAATTCCATAAATATGAAATTTAGAAAAACGAATATTAAAAAC
AAACGCAACATAGCGTGGATACTCATCTAGTGATAAGGATAAGAACTTTAGGATACTTCT
TAAGGGTTACATTTAGAACTATGAAAATATAAAATCAACTCCATTGGGTAAGGACCGGGA
TAAGCTGAAGTGGAACTTTCTTAGGACAGACAAGTCCATATGATTCTTCAAGATCAACAT
CTTCTACCTCCATTCCTTCCGGAAGCTTCCAATCAAATCGGTAAAGAAGATTGATAAGAG
TCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGANAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCT
   
Sequence Name 40-K017401-022-145-C04C-8409
GenBank Accession LN503543 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K017401-022-145-C04C-8409
GGACATGAAGCCATTTACAATTGAATATATCCTGACTCTTATCAATCTTCTTTACCGATT
TGATTGGAAGCTTCCGGAAGGAATGGAGGTAGAAGATGTTGATCTTGAAGAATCATATGG
ACTTGTCTGTCCTAAGAAAGTTCCACTTCAGCTTATCCCGGTCCTTACCCAATGGAGTTG
ATTTTATATTTTCATAGTTCTAAATGTAACCCTTAAGAAGTATCCTAAAGTTCTTATCCT
TATCACTAGATGAGTATCCACGCTATGTTGCGTTTGTTTTTAATATTCGTTTTTCTAAAT
TTCATATTTATGGAATTATGTGTAGACATTAGATTATTAAAGACTTATGTTGAGAAAGTT
AACTACAATGCTACCATATGAAATGAACATTAAAAAATAGCTGAAAGAAGTAATGTAAAA
CGGTTTTTTGAAGTGTTATGTGTCTTTACATTTAGCATGATGTACGTATCTTATGTTTTT
TTTGGATTGAGGAGAGGCATTATCATAGTTCGATTATGCAATGTAGCATTGGGAGATTTT
CAAAACTGCCGATAT

Last Updated on 06.12.2018 16:11