Sequences from confirmation amplicon


Confirmation sequences for line 145H10 and hit At1g21780 are shown.
Line ID 145H10
Locus At1g21780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7652263
Primers used for PCR FK28 - 8474, show primer details
   
Sequence Name 10-K101267-0022-145-H10C-8409
GenBank Accession LN490581 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K101267-0022-145-H10C-8409
TCGGAATGAGCCATTTACATTGTTTCTCTAGTCTAAGTCCACTCTCTTTCATTTCAACCG
AGAATTGATTTTAATATATCGTTAGTAAACCGGTTTAATGGATAAAAACGTATAATCGTC
GTACACGTGGCAGGCGATGGAGGCGGCAGAGTGTTTTGCTTGCTTCGTCGTGTTATTTGG
CGTGAAGTAAAGAGTTCAGAGAGAAAAGGAAGTCAAAACTTCAGAGAGAAAGTGGATGGG
GGACACGAAGGTAGAGACAATCTCGAGGTTAGCTCAATGGAGAATTGAAAATTTTGGTCC
TTGTTCTTTCAAAAAGTCCGATCCTTTCAAAGTCGGAATTTGGAATTGGTAAGCTTTTCA
TTTTTGGTTTATTTTGGTAAATTTTCTTCGGAATTTTGATTTTTGTTTGTTTTGGGATAT
GATGAAGGCATCTGTCTATAGAGAGGAATCGCTATCTATCAGTGAGGCTATTCCCAGAAC
TTTCTCGAGTATCTAAAGAGCAACCTCCTGTTGCCAAATTTGTTCTTCGTGTCTCCAACG
TTGGCCCCAATCGTAGATTCTACATCTCTCCTGGTATGTCTTGTCTTTGAATTGACTTTT
CTGGATTTGGAATTTGAATTCAC
   
Sequence Name 10-K101273-0022-145-H10C-FK28
GenBank Accession LN490582 [GenBank]
Sequencing primer gene specific primer, FK28: 5'-GAATTCAAATTCCAAATCCAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K101273-0022-145-H10C-FK28
GATGTAGAATCTACGATTGGGGCCAACGTTGGAGACACGAAGAACAAATTTGGCAACAGG
AGGTTGCTCTTTAGATACTCGAGAAAGTTCTGGGAATAGCCTCACTGATAGATAGCGATT
CCTCTCTATAGACAGATGCCTTCATCATATCCCAAAACAAACAAAAATCAAAATTCCGAA
GAAAATTTACCAAAATAAACCAAAAATGAAAAGCTTACCAATTCCAAATTCCGACTTTGA
AAGGATCGGACTTTTTGAAAGAACAAGGACCAAAATTTTCAATTCTCCATTGAGCTAACC
TCGAGATTGTCTCTACCTTCGTGTCCCCCATCCACTTTCTCTCTGAAGTTTTGACTTCCT
TTTCTCTCTGAACTCTTTACTTCACGCCAAATAACACGACGAAGCAAGCAAAACACTCTG
CCGCCTCCATCGCCTGCCACGTGTACGACGATNATACGTTTTTATCCATTAAACCGGTTT
ACTAACGATATATTAAAATCAATTCTCGGTTTGAAATGAAAGAGAGTGGACTTAGACTTA
GAGAAACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTA
GATGTCCG

Last Updated on 06.12.2018 16:11