Sequences from confirmation amplicon


Confirmation sequences for line 146A12 and hit At3g45555 are shown.
Line ID 146A12
Locus At3g45555 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16712916
Primers used for PCR 11cp - 8474, show primer details
   
Sequence Name 32-K109029-0022-146-A12C-11cp
GenBank Accession LN500616 [GenBank]
Sequencing primer gene specific primer, 11cp: 5'-AAGCATCTCACAAATCCATCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K109029-0022-146-A12C-11cp
ATTTCGAATTAAGCCGAGCACCTCGGGTTCGGGCAATAAATCTCTCCGTTACTGGAATGG
ATTCCTCTTCAATCTTTTGTTCCCACNATCGCTTTTAGTTTAGATGTCAAAATATGAAAA
CAACTTTTAAGAGTCAGCTTAGACTCACATTGATAATAAGGACATATCAGTACAGTTCCC
TCTAGAAGCCTCACTTCTATATATCGTTTCATGCATTCCAAACAGAATTTATGATTGCAT
AAAGCAACATATTAAAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 16-K109028-0022-146-A12C-8409
GenBank Accession LN493465 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K109028-0022-146-A12C-8409
CATTTACATTGAATATATCCTTTTAATATGTTGCTTTATGCAATCATAAATTCTGTTTGG
AATGCATGAAACGATATATAGAAGTGAGGCTTCTAGAGGGAACTGTACTGATATGTCCTT
ATTATCAATGTGAGTCTAAGCTGACTCTTAAAAGTTGTTTTCATATTTTGACATCTAAAC
TAAAAGCGATGTGGGAACAAAAGATTGAAGAGGAATCCATTCCAGTAACGGAGAGATTTT
ATTGCCCGAACCCGAGGTGCTCGGCTTTAATGTCGAAAATCGAGCTCTCTAAATCTACCT
TAGAAGATGGATTTGTGAGATGCT

Last Updated on 06.12.2018 16:11