Sequences from confirmation amplicon


Confirmation sequences for line 146C07 and hit At3g16660 are shown.
Line ID 146C07
Locus At3g16660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5677728
Primers used for PCR KB29 - 3144, show primer details
   
Sequence Name 24-K100819-0022-146-C07C-3144
GenBank Accession LN496914 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K100819-0022-146-C07C-3144
CCTTCNCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCTCACCGGCCTCCTCAACAT
CATCAGTATCATCCCTGCCGGCTTTGGTCTTCTTAACTGATTCCACACTCTATACATACT
CTATGTTCGGTTACAGTGTGTTTCCCTTTTGTTATATGTTTTGTAATCATGACCATTTAC
GTTTGCTTGCGTATGCATGAATAAGGTCTATATAGGGCTTATGTCTCAATCTTCAATGTC
TTCTGTTGTCACTTTCTTTTTCCAGGGTTTGCATGTCTAATGCTTTTGTTATATGATACA
AACAATTTCCCTTTCCAAATATATATGTCAATTGCATTAAATGAATGGTTGGGGTGAGTG
TACATGCCAAGAAGT
   
Sequence Name 24-K100831-0022-146-C07C-KB29
GenBank Accession LN496916 [GenBank]
Sequencing primer gene specific primer, KB29: 5'-TACTTCTTGGCATGTACACTCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K100831-0022-146-C07C-KB29
TTTTGGAAAGGGAATTGTTTGTATCATATAACAAAAGCATTAGACATGCAAACCCTGGAA
AAAGAAAGTGACAACAGAAGACATTGAAGATTGAGACATAAGCCCTATATAGACCTTATT
CATGCATACGCAAGCAAACGTAAATGGTCATGATTACAAAACATATAACAAAAGGGAAAC
ACACTGTAACCGAACATAGAGTATGTATAGAGTGTGGAATCAGTTAAGAAGACCAAAGCC
GGCAGGGATGATACTGATGATGTTGAGGAGGCCGGTGAGATAGTTTAAACCGAAGGCGGG
AAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATAT
AGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGAT
ATCAC

Last Updated on 06.12.2018 16:11