Sequences from confirmation amplicon


Confirmation sequences for line 146E10 and hit At2g47020 are shown.
Line ID 146E10
Locus At2g47020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19320585
Primers used for PCR 13qi - 3144, show primer details
   
Sequence Name 23-K115435-0022-146-E10C-13qi
GenBank Accession LN496683 [GenBank]
Sequencing primer gene specific primer, 13qi: 5'-GAATTCACAGGGTTCAGGTATGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K115435-0022-146-E10C-13qi
AATAATTCACACTTGAACATCTTTGCACAGCGTATTCCTATCACAGAGAAATCTGGTCGA
ATTTCACACTAGCGCTATATCTGTCGCCATCCTTCCCCAGGCTGATGAGGTACCAATGTT
TCTCCAATCTTAGTTTTTGCCACCTGTTATTAATTTTATCTTACAAGTTACATTTTTCTT
TTATTATCGTTCTTATCGGTTAATCTTACAAACGTACATACTTTTTGATCATCGTTCTTA
CTGGTGGCTACTTACAGGTAGATGTTCAGCTAAGGAATGAAGATTTGAGGATTGACACTT
ATAGGTCTGGTGGCTCAGGCGGTCAACACGCCAACACAACAAACAGCGCAGTCCGAATAA
TCCATCTTCCAACTAAGAAAATATGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTC
TCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGT
GCGTCATCCCTTACGTCAGTGGAGATA
   
Sequence Name 07-K115434-0022-146-E10C-3144
GenBank Accession LN489406 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115434-0022-146-E10C-3144
CTTCCTCTATAAAGGAAGTTCATTTCATTTGGAGCATATAGGGTACCCGGGGATCAGATT
GTCGTTTCCATATTTTCTTAGTTGGAAGATGGATTATTCGGACTGCGCTGTTTGTTGTGT
TGGCGTGTTGACCGCCTGAGCCACCAGACCTATAAGTGTCCAATCCTCAAATCTTCATTC
CTTAGCTGAACATCTACCTGTAAGTAGCCACCAGTAAGAACGATGATCAAAAAGTATGTA
CGTTTGTAAGATTAACCGATAAGAACGATAATAAAAGAAAAATGTAACTTGTAAGATAAA
ATTAATAACAGGTGGCAAAACTAAGATTGGAGAAACATTGGTACCTCATCAGCCTGGGGA
AGGATGGCAACAGATATAGCGCTAGTGTGAATTCAACCAGATTTCTCTGTGATAGGAATA
CGCTGTGCAAAGATGTTCAAGTGTGATTATTTGATTTTGTATGATTAGTCAAGAAAATCA
GAATGTGATTCATA

Last Updated on 06.12.2018 16:11