Sequences from confirmation amplicon


Confirmation sequences for line 146E10 and hit At5g48100 are shown.
Line ID 146E10
Locus At5g48100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19492683
Primers used for PCR KS53 - 8474, show primer details
   
Sequence Name 32-K101563-0022-146-E10C-8409
GenBank Accession LN500521 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K101563-0022-146-E10C-8409
CATTTACATTGAATATACCGAAAGACGGTCTCTATACGTATATAAAATATATGTATATAT
GCCTCTCTATTCTTTGATGAACTTCAGTCAGGGTAATTAATTTACTAAAACTCTTCCAAA
ATGTCACATTCCTTCTTCAATTTATTCCTAATCTCTCTTTTCCTCTACAACAATTGCATT
GCGCATCACTATACATTCACGGTAAGTTTAAATTAGTTAGTCAATTGGGCGTGAGTAAAC
AAACTTTAATTGATTAAAACTAATAAAATCGTTAATTAATGGCAGGTTAGGGAAGTTCCA
TATACGAAACTGTGTAGCACGAAGGCGATTTTAACCGTTAATAGTCAGTTTCCTGGACCG
ATTATAAAGGTTCACAAAGGAGACACTATTTATGTTAACGTTCAAAACCGAGCTAGTGAA
AA
   
Sequence Name 32-K101576-0022-146-E10C-KS53
GenBank Accession LN500522 [GenBank]
Sequencing primer gene specific primer, KS53: 5'-GTTTTCACTAGCTCGGTTTTGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K101576-0022-146-E10C-KS53
GACCTTTATATCGGTCCAGGAAACTGACTATTAACGGTTAAAATCGCCTTCGTGCTACAC
AGTTTCGTATATGGAACTTCCCTAACCTGCCATTAATTAACGATTTTATTAGTTTTAATC
AATTAAAGTTTGTTTACTCACGCCCAATTGACTAACTAATTTAAACTTACCGTGAATGTA
TAGTGATGCGCAATGCAATTGTTGTAGAGGAAAAGAGAGATTAGGAATAAATTGAAGAAG
GAATGTGACATTTTGGAAGAGTTTTAGTAAATTAATTACCCTGACTGAAGTTCATCAAAG
AATAGAGAGGCATATATACATATATTTTATATACGTATAGAGACCGTCTTTCGGTATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATNTC
CGCACGATTATT

Last Updated on 06.12.2018 16:11