Sequences from confirmation amplicon


Confirmation sequences for line 147B04 and hit At1g74110 are shown.
Line ID 147B04
Locus At1g74110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27868156
Primers used for PCR MK73 - 8474, show primer details
   
Sequence Name 22-K101424-0022-147-B04C-8409
GenBank Accession LN496048 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K101424-0022-147-B04C-8409
CATCTTTGGCCTCCTCTTGGCTTTTGTCAACAACGCCTTAACACACAGAATCCTCGCCAA
TATTGCTGACACTTGCAAAGCAAAAGCTCTCATGGCGTTCTCCGTAGGGTCAACCCGGTT
TGTTATAACCAGCGAACCAGAGACCGCGAAAGAGCTTCTAAACAGCTCTGCTTTTGCAGA
CCGGCCAGTGAAAGAGTCTGCTTACGAGCTGCTCTTTGATAGAGCCATGGGGTTTGCTCC
CTTTGGTGATTACTGGAGAGAGTTGAGGAGAATCTCTTCTACCCATCTCTTCAGCCCTAA
GAGGATCTTCAGTTCTGGCGAGTCCCGCCGAAAAATCGGGCAAAACATGGTAGGAGAGAT
CAAGAACGCANTGGAGTGTTATGGAGAAGTGCATATAAAAAAGATCTTGCATTTCGGATC
ACTCAACAACGTGATGTCTAGCGTTTT
   
Sequence Name 62-K101427-0022-147-B04C-MK73
GenBank Accession LN510715 [GenBank]
Sequencing primer gene specific primer, MK73: 5'-GTTTTACCGAAAACGCTAGACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K101427-0022-147-B04C-MK73
AGATCTTTTTATATGCCTTCTCCATAACACTCCATTGCGTTCTTGATCCCTTATACATGT
TTTGCCCGATTTTTCGGCGGGACTCGCCCTAACTGAAGATCCTCTTAGGGCTGAAGAGAT
GGGTAGAAGAGATTCTCCTCAACTCTCTCCAGTAATCACCAAAGGGAGCAAACCCCATGG
CTCTATCAAAGAGCAGCTCGTAAGCAGACTCTTTCACTGGCCGGTCTGCAAAAGCAGAGC
TGTTTAGAAGCTCTTTCGCGGTCTCTGGTTCGCTGGTTATAACAAACCGGGTTGACCCTA
CGGAGAACGCCATGAGAGCTTTTGCTTTGCAAGTGTCAGCAATATTGGCGAGGATTCTGT
GTGTTAAGGCGTTGTTGACAAAAGCCAAGAGGAGGCCAAAGATGGGGAGACCAGACGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAG

Last Updated on 06.12.2018 16:11