Sequences from confirmation amplicon


Confirmation sequences for line 147B11 and hit At4g21900 are shown.
Line ID 147B11
Locus At4g21900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11619332
Primers used for PCR BM02 - 8474, show primer details
   
Sequence Name 09-K015830-022-147-B11C-8409
GenBank Accession LN489957 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K015830-022-147-B11C-8409
TAGATCAATTCATTGCCTTCTTCATTGCTCTGCGCCGTCCCGCCTTGTCTCTCTCAAATT
CGCTTACTCATCCCTCGCAGGTTAGCTTCTTCTACCATTATTGCTTTCGCATTCCTTCTG
GGTTCGTTTCTATACCTAAAAGTTTCCGCCTATTCTAAAGTTATTGTCGATCTTAAAGTA
CAGAGATTGATAAACTCTAGTTGTTATCTTTCAATGTCGAATCATTTAACCTAGATTACT
CAGCTCTTCTACGGAATTAGTCACTAGCTTAGTTTACACAGCCTAAGAATCGGGTCCAGA
GTTTTTAGGATTCATGCATGTTCCTTAAACGCTATGTTCTGATGAAAATCTTGTGTATAT
ACTTGTTCTTTATCCCCTTTAGATACTGAGTTTAGTCACTGATCTAAATCAATTTCCTGG
TTTCAATCTCTGTGAAAAGATTGATATTTCTGAATGTTGTTGTTAAATGGTTTCTTTCTG
CTCACTTAATGTCAGGGTA
   
Sequence Name 10-K015830-022-147-B11C-BM02
GenBank Accession LN490422 [GenBank]
Sequencing primer gene specific primer, BM02: 5'-GATGAAACCCTTACCCTGACATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K015830-022-147-B11C-bm02
GAAACCATTTAACAACAACATTCAGAAATATCAATCTTTTCACAGAGATTGAAACCAGGA
AATTGATTTAGATCAGTGACTAAACTCAGTATCTAAAGGGGATAAAGAACAAGTATATAC
ACAAGATTTTCATCAGAACATAGCGTTTAAGGAACATGCATGAATCCTAAAAACTCTGGA
CCCGATTCTTAGGCTGTGTAAACTAAGCTAGTGACTAATTCCGTAGAAGAGCTGAGTAAT
CTAGGTTAAATGATTCGACATTGAAAGATAACAACTAGAGTTTATCAATCTCTGTACTTT
AAGATCGACAATAACTTTAGAATAGGCGGAAACTTTTAGGTATAGAAACGAACCCAGAAG
GAATGCGAAAGCAATAATGGTAGAAGAAGCTAACCTGCGAGGGATGAGTAAGCGAATTTG
AGAGAGACAAGGCGGGACGGCGCAGAGCAATGAAGAAGGCAATGAATTGATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11