Sequences from confirmation amplicon


Confirmation sequences for line 147G11 and hit At5g47480 are shown.
Line ID 147G11
Locus At5g47480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19258455
Primers used for PCR 02do - 8474, show primer details
   
Sequence Name 31-K109936-0022-147-G11C-02do
GenBank Accession LN500205 [GenBank]
Sequencing primer gene specific primer, 02do: 5'-GTGGGTTTGGTTCATACTCTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K109936-0022-147-G11C-02do
CGGAGGTAGCTGTGGCCACTGGAGGAAACTTAGTAGCCAATGATACAATTAACACAAGTG
TTGGTTTAGATAATTCGGCAGGCTTTGAGCAACACCAGGGTCAAGTGCAACACGATTCTG
GAAGCGGGCAGTATGTGGATAACAGTCAATCTTGGGAAAATCTCTATCCTGGATGGAAAT
ATGATGCAAGTACTGGTCAATGGTATCAAGTTGATGGTCAGGATGCAACCGTTAATTCAC
AGGAGAGCTATATTAACTCAACAGGTAACTGGGAAAGCGTTGCTGCTGATAACTCGGATG
TTGCTTATCTGAAACAGAGTACAACATCTGCAATGGCTGGCACAGCTGAGAGTGTGTCTA
CATGGAACCAGGTTTCACAAGTGGGCAATGGATATCCAGAACACATGGTATTTGACGCCC
AGTATCCTGGATGGTACTATGACACAATTGCTCAAGAATGGCGCTCTCTGGACAGCTACA
ACCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACCTGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAATTATTACCAATTTTTTTTCAATTCAA
AAAT
   
Sequence Name 15-K109935-0022-147-G11C-8409
GenBank Accession LN493017 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K109935-0022-147-G11C-8409
GCCATTTACAATTGAATATATCCTGGTTGTAGCTGTCCAGAGAGCGCCATTCTTGAGCAA
TTGTGTCATAGTACCATCCAGGATACTGGGCGTCAAATACCATGTGTTCTGGATATCCAT
TGCCCACTTGTGAAACCTGGTTCCATGTAGACACACTCTCAGCTGTGCCAGCCATTGCAG
ATGTTGTACTCTGTTTCAGATAAGCAACATCCGAGTTATCAGCAGCAACGCTTTCCCAGT
TACCTGTTGAGTTAATATAGCTCTCCTGTGAATTAACGGTTGCATCCTGACCATCAACTT
GATACCATTGACCAGTACTTGCATCATATTTCCATCCAGGATAGAGATTTTCCCAAGATT
GACTGTTATCCACATACTGCCCGCTTCCAGAATCGTGTTGCACTTGACCCTGGTGTTGCT
CAAAGCCTGCCGAATTATCTAAACCAACACTTGTGTTAATTGTATCATTGGCTACTAAGT
TTCCTCCAGTGGCCACAGCTACCTCCGCCTGTCCCTGTACATTTCCTGCAGTTGCATCCA
ACTCAGTGAAGAAATCAGAGTATGAACCAAACC

Last Updated on 06.12.2018 16:11