Sequences from confirmation amplicon


Confirmation sequences for line 148B05 and hit At3g02000 are shown.
Line ID 148B05
Locus At3g02000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:332832
Primers used for PCR RE02 - 3144, show primer details
   
Sequence Name 21-K023833-022-148-B05C-3144
GenBank Accession LN495496 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K023833-022-148-B05C-3144
GGATGACGCACAATCCCACTATCCCCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTT
CATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCTCTATATACTCATGA
AGCAGACCAGTGTCAGCCACCATCCCCAAACCGCCGAATCCTAAGCTGCTCATCTTGTAT
GATCCCCACGATTCTGTCTGGTATTGCATCTTTGATATCTCTTCTCTTTCTCTTGTTACA
CTTTTGTTTGGAAAGAAACTAGATTAAACACGAATAAGAGAGAAAGAGGGATTGGCTAAA
GTTTGTTGGTGAGAAGAGAGTGAGAGCCATGCAGAGTATTTATAGAGGCCTACTACACCT
CTGAGTCTAACTCTGTTTTCCTCTAATTATTAGTATTATTGTTTCTTTCTTTATTATTTG
TGTATAATAACATGACCTCTATTTACTTCTCCATGAAAAAAAGAAAAAAAGTAACAAAAC
ATATGAATTAAAACCGATGACATCAAGATAGTGATTGCTGTTGAAAACAAACACCGATAA
TAGTTATCGGAATAATTTCATTTTTTTGTAAAAAAAAAAAAAAAGGCAGGGAGAGTTGAA
AAACAGGTTTTTATCAAAAGGGAGTTGTTGGAAAAA
   
Sequence Name 25-K023833-022-148-B05C-RE02
GenBank Accession LN497269 [GenBank]
Sequencing primer gene specific primer, RE02: 5'-AATTAATGGTGAGAGCGGTAAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K023833-022-148-B05C-RE02
GACAGACCGAGCCTTCTATTACCAACAACTCCCTTCTGATAAAAGCCTGTTCTTCAACTC
GCCTTGCCTTTTTTTTTTTTTTAACAAAAAAAGGAATCTATTCCCATAACTTTGATCGGG
GTTTGTTTACAACAGCAACCCCTATTTGGATGCCATCGGTTTAAATTCATATGTTTTGTT
CCTTTTTTTCTTTTTTCCGAGGAAAAGAAAAGAAAGGTCTTGTTTTTCACCCCAAAAAAA
AAAAAAGGAAACAATAATCCAAAAAATGAAAAAAAAACAAATTTAAACCCAGAGGGGGGC
CAGCCCCCAAAAAATACCGGGGGGGGCTCTCCCTCTCTCCAAAAAAAAAAATTTAACCCA
ACCCCCCTTTCTCTCTAAGTCGGGTTAAATCCCTTTCCTTCCCAAACAAAAGGGTACCAA
GGAAAAGAGAAAAAATTTCAAAGTTCCCACCCCAAACCGAGGCGGGGGGACCAAACAAAA
AGACCACCTTAGGATCCGGCGGTTTGGGGAGGGGG

Last Updated on 06.12.2018 16:11