Sequences from confirmation amplicon


Confirmation sequences for line 149A05 and hit At3g10340 are shown.
Line ID 149A05
Locus At3g10340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3206783
Primers used for PCR MS37 - 8474, show primer details
   
Sequence Name 31-K017447-022-149-A05C-MS37
GenBank Accession LN499947 [GenBank]
Sequencing primer gene specific primer, MS37: 5'-GGCTGTTTTATCGGAAGTTATGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K017447-022-149-A05C-MS37
TGCCATGTTCGCAGAGGTTATGCAAGGGAAACCGGAGTTTACAGATCATCTTACGCATAA
GCTCAAGCACCATCCCGGTCAGATCGAAGCCGCCGCAATTATGGAACATATATTAGACGG
AAGCTCTTACGTTAAAGAAGCTCAACTTCTCCACGAAATGGATCCTCTTCAAAAACCTAA
ACAAGATCGGTAATGTATCTTGTTCTTCAAGTAAACTGATATTGTGTGTTGAAGTTGATG
TTTTCGTGTGTGATGATTATATTAGCTGAGTAGTGTTTCCTTTTAAGTTGCAGGTACGCT
TTACGTACGTCACCACAATGGCTTGGGCCGCAGATTGAAGTGATCAGAGCGGCTACTAAA
ATGATTGAGTTGATCGCCTTGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 32-K017447-022-149-A05C-8409
GenBank Accession LN500374 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K017447-022-149-A05C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAACAAGGCGATCAACTCAAT
CATTTTAGTAGCCGCTCTGATCACTTCAATCTGCGGCCCAAGCCATTGTGGTGACGTACG
TAAAGCGTACCTGCAACTTAAAAGGAAACACTACTCAGCTAATATAATCATCACACACGA
AAACATCAACTTCAACACACAATATCAGTTTACTTGAAGAACAAGATACATTACCGATCT
TGTTTAGGTTTTTGAAGAGGATCCATTTCGTGGAGAAGTTGAGCTTCTTTAACGTAAGAG
CTTCCGTCTAATATATGTTCCATAATTGCGGCGGCTTCGATCTGACCGGGATGGTGCTTG
AGCTTATGCGTAAGATGATCTGTAAACTCCGGTTTCCCTTGCATAACCTCTGCGAACATG
GCAGACATAACTTCCGATAAAACAG

Last Updated on 06.12.2018 16:11