Sequences from confirmation amplicon


Confirmation sequences for line 149C09 and hit At5g21150 are shown.
Line ID 149C09
Locus At5g21150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7198014
Primers used for PCR KD03 - 8474, show primer details
   
Sequence Name 45-K017122-022-149-C09C-KD03
GenBank Accession LN505359 [GenBank]
Sequencing primer gene specific primer, KD03: 5'-AAATTTGGGTTTTACGGTTTTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K017122-022-149-C09C-KD03
CCTGAAGGGTTACTTAACTAACACACCCTGAAGGTTATAAGTACCTAAAGTTGCAAATTT
TCTGGGCAAAATGTTTTCTTTTAAACCTAGCAGATGTTGTAGTTCATCGATATGTTCCAA
ATCTAACTATATGATGCAAATCACACTGCCTATATGTAATTCAGCCACAACATCAGATCG
ACGTTAAAAAAAGAAGAGAACTCAATGCCTTAAAATACCAAATCTGATTAAGCATAATAA
AGTATCAACCAACAGCGCCCACAAAAACCGACAGAATATAAAGAGTATCTCCTCAATGTT
AAAGGGTCGACGAAAAACAAAAGAGGAACCAAACCCAGAGAAGAGTAAACAAAAGTTCGT
TGGTTCTCCATCAAAAGCATATAAACTCAGCTATCGTGTGGACTGGACCATGTTCCATCA
ACAGAAGAACATGGAGGTTGAAACATTATTGTGCAGCTGCGGCATAGGTGGCACAGGGAC
TGCTCCAGGTGTGGTGATTCCTCCATGGCTCGAAGAAGTCTCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAA
   
Sequence Name 46-K017122-022-149-C09C-8409
GenBank Accession LN505709 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K017122-022-149-C09C-8409
TTCTTCGAGCCATGGAGGAATCACCACACCTGGAGCAGTCCCTGTGCCACCTATGCCGCA
GCTGCACAATAATGTTTCAACCTCCATGTTCTTCTGTTGATGGAACATGGTCCAGTCCAC
ACGATAGCTGAGTTTATATGCTTTTGATGGAGAACCAACGAACTTTTGTTTACTCTTCTC
TGGGTTTGGTTCCTCTTTTGTTTTTCGTCGACCCTTTAACATTGAGGAGATACTCTTTAT
ATTCTGTCGGTTTTTGTGGGCGCTGTTGGTTGATACTTTATTATGCTTAATCAGATTTGG
TATTTTAAGGCATTGAGTTCTCTTCTTTTTTTAACGTCGATCTGATGTTGTGGCTGAATT
ACATATAGGCAGTGTGATTTGCATCATATAGTTAGATTTGGAACATATCGATGAACTACA
ACATCTGCTAGGTTTAAAAGAAAACATTTTGCCCAGAAAATTTGCAACTTTAGGTACTTA
TAACCTTCAGGGTGTGTTAGTTAAGTAACCCTTCAGGAATATTGGTAAAACCGTAA

Last Updated on 06.12.2018 16:11