Sequences from confirmation amplicon


Confirmation sequences for line 149H10 and hit At2g05710 are shown.
Line ID 149H10
Locus At2g05710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:2146540
Primers used for PCR ME13 - 8474, show primer details
   
Sequence Name 25-K027344-022-149-H10C-8409
GenBank Accession LN497292 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K027344-022-149-H10C-8409
GCCATTTACAATTGAATATTATTGGTTTTTGAAAAGGAATAAGAGAGCCTTCTTGCATAT
TCCCATTACTGACTCCCAAAGACCTCGCAATCTGTTGCATTTCACAAGCGTATTCAGCAG
CATCTATACCAATTCTCAGATCATGTTCAAGAACACCTGCTCTATTCAAATCTACTTGCA
CCATTAGAGTAACCAACTAATACATAAGAAACACAAACATCTTGCAGCTGAAGCCTAAAA
CCAATACAAGAATCTGATAAAACCAAACTGAAACTTGTCATAAATTCCATATGAACCTGA
GAGTAATCTCCTGTGTCTGAATGAAGTAACTTGCCAGCATCTTCTTCCATATGAACCCTA
GTAATACCAAAATCTCCTATGTCCACCACCAAACTCAAGAGGAATATCCAAATCTGATAA
CCTTGAAGAAGATCTGGATAAAAATACTGCTTCCTATCGAATTTAGACTTAAGAGACAAA
TCACAATTCAAAGCTAACCCCAATAACCCACTTTTTTAATAGATTTCCTTTCTTTCT
   
Sequence Name 26-K027344-022-149-H10C-ME13
GenBank Accession LN497766 [GenBank]
Sequencing primer gene specific primer, ME13: 5'-TGGATCAGAAAGAAAGAAAGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K027344-022-149-H10C-ME13
GGTATTGGGGTAGCTTTGATTGTGATTTGTCTCTTAAGTCTAAATTCGATAGGAAGCAGT
ATTTTTATCCAGATCTTCTTCAAGGTTATCAGATTTGGATATTCCTCTTGAGTTTGGTGG
TGGACATAGGAGATTTTGGTATTACTAGGGTTCATATGGAAGAAGATGCTGGCAAGTTAC
TTCATTCAGACACAGGAGATTACTCTCAGGTTCATATGGAATTTATGACAAGTTTCAGTT
TGGTTTTATCAGATTCTTGTATTGGTTTTAGGCTTCAGCTGCAAGATGTTTGTGTTTCTT
ATGTATTAGTTGGTTACTCTAATGGTGCAAGTAGATTTGAATAGAGCAGGTGTTCTTGAA
CATGATCTGAGAATTGGTATAGATGCTGCTGAATACGCTTGTGAAATGCAACAGATTGCG
AGGTCTTTGGGAGTCAGTAATGGGAATATGCAAGAAGGCTCTCTTATTCCTTTTCAAAAA
CAATAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTA

Last Updated on 06.12.2018 16:11