Sequences from confirmation amplicon


Confirmation sequences for line 150D04 and hit At3g47160 are shown.
Line ID 150D04
Locus At3g47160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17361104
Primers used for PCR KS83 - 8474, show primer details
   
Sequence Name 33-K101564-0022-150-D04C-8409
GenBank Accession LN500934 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K101564-0022-150-D04C-8409
GCCATTTACATTGAATATATCCTGAACACGCGCTCCATCCTTCTCCCGAGGATAATCTAA
AGCTCTGTTAATTCACAGAGGAGAAACAAGTGACAATGTTCATAAGCACCATAACAACAT
CACTTGATGAAAAAACACAAACATAACAAAATTCATCATTCAATGTATTGAACGAATCAC
AGAGATTATAAACAACAGAAGCAATTGATCAATGCACCATTGAGATGCATGGAAGTGAGG
AACTTAAAGATAGCATCACAAGTACACCTCTTGATCATTGCAGAAACACATTTTCAGCTT
CAAAGATCAAAGTTTGATTTAATCAAGCATCCTCATATGTCTAAATTGATTAAGAAACTC
ACCAAAACATTCACCTTTTAGACATAGAACTTCCTAACTCAACTAGATTACCATCAGAAA
TCAAATCAAATAAAGGTTTTAGCTTTCAAATATTTGATCATTTCAGACAAACACAGTTTC
CTCTGAGATCATCAAGCCAAAGACTTTGTTCAGCTTCAAGAGTCAACACTCAAACTAGGA
CAAGACTACCTAAAAATGACTTCTCCCCCCAATT
   
Sequence Name 33-K101577-0022-150-D04C-KS83
GenBank Accession LN500935 [GenBank]
Sequencing primer gene specific primer, KS83: 5'-GAATTGGGTGATGAAGTCATTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K101577-0022-150-D04C-KS83
TGACTCTGAGCTGAACAAGTCTTTGGCTTGATGATCTCAGAGGAAACTGTGTTTGTCTGA
AATGATCAAATATTTGAAAGCTAAAACCTTTATTTGATTTGATTTCTGATGGTAATCTAG
TTGAGTTAGGAAGTTCTATGTCTAAAAGGTGAATGTTTTGGTGAGTTTCTTAATCAATTT
AGACATATGAGGATGCTTGATTAAATCAAACTTTGATCTTTGAAGCTGAAAATGTGTTTC
TGCAATGATCAAGAGGTGTACTTGTGATGCTATCTTTAAGTTCCTCACTTCCATGCATCT
CAATGGTGCATTGATCAATTGCTTCTGTTGTTTATAATCTCTGTGATTCGTTCAATACAT
TGAATGATGAATTTTGTTATGTTTGTGTTTTTTCATCAAGTGATGTTGTTATGGTGCTTA
TGAACATTGTCACTTGTTTCTCCTCTGTGAATTAACAGAGCTTTAGATTATCCTCGGGAG
AAGGATGGAGCGCGTGTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATATCCGCA

Last Updated on 06.12.2018 16:11