Sequences from confirmation amplicon


Confirmation sequences for line 150D10 and hit At5g65260 are shown.
Line ID 150D10
Locus At5g65260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26082058
Primers used for PCR 25ra - 8474, show primer details
   
Sequence Name 81-K115895-0022-150-D10C-25ra
GenBank Accession LN514107 [GenBank]
Sequencing primer gene specific primer, 25ra: 5'-AATACTCCACTCAAACATTGTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K115895-0022-150-D10C-25ra
CATTTTTCCTTCTCTATATTGCTCTAAGCGTATTCATTTCCTTTTCTTTATTCTTTTTTC
ATAGCTTTATTCAATGCTTTTTTGCCTCTTTTTTTTAGCTTTTTTCATCATGTTCCACAA
CAAAACAGAACAGATCTTTTTCAACTCCATAAAAGGAAAATGTAAAGACCCCACAAAACC
TAATTGATATAACCCAGAGCAATTAAGAACTTCAGGAACAAAATCCTGCGATTGTAGTGA
GAAAAATCATAAGAAAAGTAAAACTCAACGCCAGAAAACAAAAACTATAGCATCCTCAAA
TTTGCACAGAAAAGTTACTAAACATATGAAATTTTCAATTATATCTGAAGGCTAAGGTTC
CACCAGTAATATGAAGACTCAATCTACTTTCCAAAACCCTCAATATCACTAACAACAGAA
ACCCCTCACTAGTTTTCATTTGAAGATAAAGTCTTCACGCTAAGCACCAACTTTTTACCA
TTCAACAATTTCATCTAATAAGAGTTCTCACTGAATAGACNAGAATCCATAGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAAAGTAGATATCCGCA
   
Sequence Name 51-K115894-0022-150-D10C-8409
GenBank Accession LN507605 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K115894-0022-150-D10C-8409
GGATTCTTGTCTATTCAGTGAGAACTCTTATTAGATGAAATTGTTGAATGGTAAAAAGTT
GGTGCTTAGCGTGAAGACTTTATCTTCAAATGAAAACTAGTGAGGGGTTTCTGTTGTTAG
TGATATTGAGGGTTTTGGAAAGTAGATTGAGTCTTCATATTACTGGTGGAACCTTAGCCT
TCAGATATAATTGAAAATTTCATATGTTTAGTAACTTTTCTGTGCAAATTTGAGGATGCT
ATAGTTTTTGTTTTCTGGCGTTGAGTTTTACTTTTCTTATGATTTTTCTCACTACAATCG
CAGGATTTTGTTCCTGAAGTTCTTAATTGCTCTGGGTTATATCAATTAGGTTTTGTGGGG
TCTTTACATTTTCCTTTTATGGAGTTGAAAAAGATCTGTTCTGTTTTGTTGTGGAACATG
ATGAAAAAAGCTAAAAAAAAGAGGCAAAAAAGCATTGAATAAAGCTATGAAAAAAGAATA
AAGAAAAGGAAATGAATACGCTTTAGAGCAATATAGAGAAGGAAAAATGTTATGATTATG
AGGGAAAAAGAGATATCTGGAGAGGGGACAATGTTTGAGTGGGAGTA

Last Updated on 06.12.2018 16:11