Sequences from confirmation amplicon


Confirmation sequences for line 151C09 and hit At2g27190 are shown.
Line ID 151C09
Locus At2g27190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11622216
Primers used for PCR ML67 - 8474, show primer details
   
Sequence Name 26-K100811-0022-151-C09C-8409
GenBank Accession LN497835 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K100811-0022-151-C09C-8409
CATTTACAATTGAATATATCCTGACGCCCTTTTGATGCCCTTTTGATGGAATACCACAAT
GGAGAGATACTTCCTGATGCCTTATGTGGCGTATGATACCGATTCATAAAAGGCTTGAAT
GGTTCTATTTCACCCTACAAAACAGACTCTTCATGTTAAGTTTAAGTAATTACCCTAGAA
GTGATAAAAGAAAGGAAGAAATTTAAATATTACAATGTCAGGAACGAAGTCGATTTCGTG
GTTGCCAGCAGTCCATATCCAAGGTTGATAAGCAACACTTCGCTCAACGAACCTTCCCCA
AGTATCCCATCTATTGTTGTCGTGGTTAGGGTAGCGATCCGCGTAAGACAAGTCTCCTAC
AAACAGAACCGCTTGTCCTTTTCCCGGATTCATCTCATAATGGCTTAACGTACTATTTGA
GTCATAAGTTTGTCCTAGATCCCCT
   
Sequence Name 26-K100825-0022-151-C09C-ML67
GenBank Accession LN497837 [GenBank]
Sequencing primer gene specific primer, ML67: 5'-CAGGGGATCTAGGACAAACTTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K100825-0022-151-C09C-ML67
GAGATGATCCGGGAAAGGACAAGCGGTTCTGTTTGTAGGAGACTTGTCTTACGCGGATCG
CTACCCTAACCACGACAACAATAGATGGGATACTTGGGGAAGGTTCGTTGAGCGAAGTGT
TGCTTATCAACCTTGGATATGGACTGCTGGCAACCACGAAATCGACTTCGTTCCTGACAT
TGTAATATTTAAATTTCTTCCTTTCTTTTATCACTTCTAGGGTAATTACTTAAACTTAAC
ATGAAGAGTCTGTTTTGTAGGGTGAAATAGAACCATTCAAGCCTTTTATGAATCGGTATC
ATACGCCACATAAGGCATCAGGAAGTATCTCTCCATTGTGGTATTCCATCAAAAGGGCAT
CAAAAGGGCGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCC
Primers used for PCR ML68 - 8474, show primer details
   
Sequence Name 27-K100811-0022-151-C09C-OriL
GenBank Accession LN498298 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K100811-0022-151-C09C-OriL
GCCTTTACAATTGAATATATCCTGAAGAAACAATAATATATTTAGTAGCTTGTTGATTAG
TTTTGCATATTATGATTAGGGATATACACACCTCAATACAAATGGCTGGAAAAGGAGTTA
CAAGGAGTGAATCGAACAGAGACGCCATGGCTTATAGTCTTAGTGCATAGCCCGTTTTAC
AGTAGCTATGTGCATCATTACATGGAAGGTGAAACATTGAGAGTCATGTACGAACAATGG
TTTGTCAAATACAAGGTTGATGTTGTGTTTGCAGGTCATGTTCATGCCTATGAGAGATCG
GTATATTCAATTTACTTGTCCCGAATAATATTGATATTTATCTAAACCTTCCTTGTCCCC
CAATTGATCCTAAAATATTGACGCAGGAACGTGTTTCTAATATTGCTTACAACATTGTCA
ATGGATTGTGTGAGCCAATATCGGATGAGTCAGCTCCTATATACATCACAATCGGGGATG
GCGGAAACTCGGAAGGCTTGCTTACCGAGTAAGTTTTACCATATTCTGCAATCAATAATA
GAGTGACTAACTTGTAACCGACTCTGTAACTAACTCTGTTATTGATAATACTCTTCACTT
TCTTTGAATATGTATGTGACTGGCTTTTGCTATATCTGTAGCATGATGCAGCCACAACCA
AAATACTCCGCGTTTCGAGAAGCTAGCTTTGGGCACGGGTTGCTAG
   
Sequence Name 27-K100825-0022-151-C09C-ML68
GenBank Accession LN498300 [GenBank]
Sequencing primer gene specific primer, ML68: 5'-CTGTTTTTAATCTCTAGCAACCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K100825-0022-151-C09C-ML68
CAAAGAAAGTGAAGAGTATTATCAATAACAGAGTTAGTTACAGAGTCGGTTACAAGTTAG
TCACTCTATTATTGATTGCAGAATATGGTAAAACTTACTCGGTAAGCAAGCCTTCCGAGT
TTCCGCCATCCCCGATTGTGATGTATATAGGAGCTGACTCATCCGATATTGGCTCACACA
ATCCATTGACAATGTTGTAAGCAATATTAGAAACACGTTCCTGCGTCAATATTTTAGGAT
CAATTGGGGGACAAGGAAGGTTTAGATAAATATCAATATTATTCGGGACAAGTAAATTGA
ATATACCGATCTCTCATAGGCATGAACATGACCTGCAAACACAACATCAACCTTGTATTT
GACAAACCATTGTTCGTACATGACTCTCAATGTTTCACCTTCCATGTAATGATGCACATA
GCTACTGTAAAACGGGCTATGCACTAAGACTATAAGCCATGGCGTCTCTGTTCGATTCAC
TCCTTGTAACTCCTTTTCCAGCCCTTTGTATTGAGGTGTGTATATCCCTAATCATAATAT
GCAAAACTAATCAACAAGCTACTAAATATATTATTGTTTCTTCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTCAAAAATGTAGA

Last Updated on 06.12.2018 16:11