Sequences from confirmation amplicon


Confirmation sequences for line 151H01 and hit At3g54270 are shown.
Line ID 151H01
Locus At3g54270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20089721
Primers used for PCR RZ14 - 8474, show primer details
   
Sequence Name 21-K018601-022-151-H01C-RZ14
GenBank Accession LN495464 [GenBank]
Sequencing primer gene specific primer, RZ14: 5'-CCAAAGTTAGACCCAAAAGAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K018601-022-151-H01C-RZ14
TTGATAACTTCTTACCACTATAATTGAAAAATTATTATTAATAGTTACCAATCATAAAAA
CGATAACAAATAATAGAACCAGAACAATATATATTTTTAATATCGTTTTTTGTATCACTA
CATACATCTCTAACAATTTTGGTATTGTTTATGATTCTCTATTTCCACTCAAACTAAATC
GATTTTTTGTCACCAATGGACTTACCAAGCAAATTCTCTTGCACATTTTCTCTGAATATA
AAATGAATGATAAGGAAGAGTCATCCATTGCTAGTAATGCATCATGTGGTGGTCGTCAGG
ATCTATCATGTCGTAATAACGCACGCATAAAACTGAATTTTGAGGGGGAACTTCGTTGCG
TTGACATGTTATATAAATACGTGCTTAAGGAAATTTCTAACTAATTAAAAAAAGAAAGAT
TATAACTAATTCAAATACTCGACAGAATTGTTCGAATAAGAAAAGAAAAACTTCGTCTTC
CCAAGGAAGTCAAGAATAAAGGAACCAAAAAGTTAGTTGAGCAAAGAGACAAGAGAGAAG
CAAAGAGAAACACCTGGGTTCCTGAAATCGATGGATAGGCTTGAAAATCCCGCCAATATA
TACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
   
Sequence Name 22-K018601-022-151-H01C-8409
GenBank Accession LN495911 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K018601-022-151-H01C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTATATATTGGCGGGAT
TTTCAAGCCTATCCATCGATTTCAGGAACCCAGGTGTTTCTCTTTGCTTCTCTCTTGTCT
CTTTGCTCAACTAACTTTTTGGTTCCTTTATTCTTGACTTCCTTGGGAAGACGAAGTTTT
TCTTTTCTTATTCGAACAATTCTGTCGAGTATTTGAATTAGTTATAATCTTTCTTTTTTT
AATTAGTTAGAAATTTCCTTAAGCACGTATTTATATAACATGTCAACGCAACGAAGTTCC
CCCTCAAAATTCAGTTTTATGCGTGCGTTATTACGACATGATAGATCCTGACGACCACCA
CATGATGCATTACTAGCAATGGATGACTCTTCCTTATCATTCATTTTATATTCAGAGAAA
ATGTGCAAGAGAATTTGCTTGGTAAGTCCATTGGTGACAAAAAATCGATTTAGTTTGAGT
GGAAATAGAGAATCATAAACAATACCAAAATTGTTAGAGATGTATGTAGTGATACAAAAA
ACGATATTAAAAATATATATTGTTCTGGTTCTATTATTTGTTATCGTTTTTATGATTGGT
AACTATTAATAATAATTTTTCAATTATAGTGGTAAGAAGTTATCAATTAAAGTCTTGTTC
T

Last Updated on 06.12.2018 16:11