Sequences from confirmation amplicon


Confirmation sequences for line 152E12 and hit At1g35730 are shown.
Line ID 152E12
Locus At1g35730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:13228798
Primers used for PCR JC13 - 8474, show primer details
   
Sequence Name 89-K101673-0022-152-E12C-8409
GenBank Accession LN514938 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K101673-0022-152-E12C-8409
GGACTGAAGCCATTTACAATTGAAAACAGATTCTGATAAGCTCCCTTGGATTTGACGTTA
ACACACTCACAATCAACGTCCTCTGTTCCTCATCACTCACATCAAACAGCTTCTGAACAA
TGTAATTCCCAAAAGGATCCATGGAAAGTTCAACGACATGGTCAATGATTGCAAGTAATA
TAACCTTAGCTTCATGAAATGTACCTTCTTCGACCAGTTTCTGCAAAACTCTGCAACTTA
TCTGATCTTTCGCCATCAAGTTCTGCGATCTATAATTCTCAACCACGGAAACGAGA
   
Sequence Name 89-K101680-0022-152-E12C-JC13
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, JC13: 5'-CTTAGATCAGATCCATGAACCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K101680-0022-152-E12C-JC13
CACGATGCTCTCATAGTTCGTTGATGTTTGAAGGAAATAATAGGGGTTTCTCAGACCCCT
AGCCGCCATGGATAGCTT

Last Updated on 06.12.2018 16:11