Sequences from confirmation amplicon


Confirmation sequences for line 153C03 and hit At2g41790 are shown.
Line ID 153C03
Locus At2g41790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17433087
Primers used for PCR 36ci - 8474, show primer details
   
Sequence Name 53-K107936-0022-153-C03C-36ci
GenBank Accession LN508178 [GenBank]
Sequencing primer gene specific primer, 36ci: 5'-CTCTCAGCTATTTGTGAGACGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K107936-0022-153-C03C-36ci
TGTCCTATATGTGGATATTGCATCAAACATGCAGGTAATAATTTAGCTCTTTCTCTTTTA
CCTAAGCTTTATTTTTTTATACATGCTTACATCTGTTACTATGTCATCAATCATTCCTTC
AATAAATCTTGCATATAAAGTTATGCAATGTGATTAAAGTTTTGTAATTTATATCTTTAA
AGCGCAAACCTAAGCTCACGGCTAAGAAATAATAGTTCTTTTAATATCTTCTGTATACTT
GGTTAAAGTTATGCAATGTGATTAAAGTTTCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 37-K107935-0022-153-C03C-8409
GenBank Accession LN502640 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K107935-0022-153-C03C-8409
GCCATTTACATTGAATATATCCTGAAACTTTAATCACATTGCATAACTTTAACCAAGTAT
ACAGAAGATATTAAAAGAACTATTATTTCTTAGCCGTGAGCTTAGGTTTGCGCTTTAAAG
ATATAAATTACAAAACTTTAATCACATTGCATAACTTTATATGCAAGATTTATTGAAGGA
ATGATTGATGACATAGTAACAGATGTAAGCATGTATAAAAAAATAAAGCTTAGGTAAAAA
GAGAAAGAGCTAAATTATTACCTGCATGTTTGATGCAATATCCACTATATAGGACATTGG
TGGTATTTTGTCTTGATAATGAAATTTCGTCTCACAAATAGCTGAGAG
Primers used for PCR CP32 - 8474, show primer details
   
Sequence Name 13-K119005-0022-153-C03C-8409
GenBank Accession LN492274 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K119005-0022-153-C03C-8409
CATTTCATTGAATATATCCTGAAACTTTAATCACATTGCATAACTCTAACCAAGGATTGG
CTGGTTGGATCATCACTACCTACTAAATTTAATCCAGCTATTGTACAAAAGGTTGTAGAT
GAGCTTTCTCCTAGTAATTTTCGGTAAGTCTGTGGTTTTAAGAGTTTTACTTTGCATACA
TAAAACTTATGGGTAGCTGCCTATTGTACACTAAACTTATAATGAAATCATTGGTTTTCG
CCTAAGCTAATTTTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGAAGGGGAA
ATTTTTCCCTTTTTCCTTTTTTTTAAAAAAAAAAGGGGGGGCCCCAAAAAATTTTTTTTT
TTTTTTTAAATAAGGGGGGAATTTTTTTTTTTTTCCCCCTTTTTTTTTTGGGAAAAAAAA
AAA

Last Updated on 06.12.2018 16:11