Sequences from confirmation amplicon


Confirmation sequences for line 153G01 and hit At1g03400 are shown.
Line ID 153G01
Locus At1g03400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:843438
Primers used for PCR BJ88 - 8474, show primer details
   
Sequence Name 09-K104856-0022-153-G01C-BJ88
GenBank Accession LN490166 [GenBank]
Sequencing primer gene specific primer, BJ88: 5'-CAGTTTTCATTGTGTACCGAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K104856-0022-153-G01C-BJ88
AACCCAATGTCTTAGATTTTGAAATGGTGTAACTTAGGAGCATCGAGCGTTTTTGAGCTG
AAGATGGTTGAAAATTCGGTGAGGGTGCAGTCTCTATACTTAGCAGGGTTTTCTGCAGAC
AGGAGATCTTTGATTGGCCCATATACTCGAGAATACGCCCTCATGAACGTGCTGAAAACA
ATTGCCACGGAAGTCCGCGGTTCAGAAGATCCATTGGCTATCACCCTATGCTCCGCGCTT
ATGAACTTATCATTGGTTATAAGCTGCACACAGTAAAAAAGAAGGATCAAACCCCAAATC
ATACATGAAATGAAGCAAGATCGAAGTAGCAACATGATCTTTTTAACCTGAAGAAAATCT
CCAATGTTAATGACTAGAGCCCCAGGGACAGGAGTAACATCAATCCAATACTGTTCATGG
AAAACTTGAAGCCCTCCAACATTGTCTTGAAGAAGAACGGTGAGAAAGGAAATATCGGTG
TGCTTGTTTATGCCTATAGTAAGGTCAGGCTGAGGGCAAGGTGGGTAGTGTTGGCCAACC
ATATATTGAGACTTGCATGAATATACAATATGTCCGAAAAAGAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 25-K104857-0022-153-G01C-8409
GenBank Accession LN497432 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K104857-0022-153-G01C-8409
TTTCTTTTTCGGACATATTGTATATTCATGCAAGTCTCAATATATGGTTGGCCAACACTA
CCCACCTTGCCCTCAGCCTGACCTTACTATAGGCATAAACAAGCACACCGATATTTCCTT
TCTCACCGTTCTTCTTCAAGACAATGTTGGAGGGCTTCAAGTTTTCCATGAACAGTATTG
GATTGATGTTACTCCTGTCCCTGGGGCTCTAGTCATTAACATTGGAGATTTTCTTCAGGT
TAAAAAGATCATGTTGCTACTTCGATCTTGCTTCATTTCATGTATGATTTGGGGTTTGAT
CCTTCTTTTTTACTGTGTGCAGCTTATAACCAATGATAAGTTCATAAGCGCGGAGCATAG
GGTGATAGCCAATGGATCTTCTGAACCGCGGACTTCCGTGGCAATTGTTTTCAGCACGTT
CATGAGGGCGTATTCTCGAGTATATGGGCCAATCAAAGATCTCCTGTCTGCAGAAAACCC
TGCTAAGTATAGAGACTGCACCCTCACCGAATTTTCAACCATCTTCAGCTCAAAAACGCT
CGATGCTCCTAAGTTACACCATTTCAAAATCTAAGACATTTGGGTTTAGACCTTTTTTGT
CTACTAATCTATCAAACTCTCTCGGT

Last Updated on 06.12.2018 16:11