Sequences from confirmation amplicon


Confirmation sequences for line 153H11 and hit At4g11350 are shown.
Line ID 153H11
Locus At4g11350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6903111
Primers used for PCR 26by - 8474, show primer details
   
Sequence Name 74-K108065-0022-153-H11C-26by
GenBank Accession LN513088 [GenBank]
Sequencing primer gene specific primer, 26by: 5'-CTAAAGAGATCGGTTTTCACCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K108065-0022-153-H11C-26by
TGGTATTTTTGTGAATGGGATTTTGCTTAGATTGGTTTGATTATAAATGATTTTGGGTTA
GAATGATTTAAAAAGGTAGTGAAATAGGCAAATTGATGATTAGTTTAGTGTGTACGTAAT
TGATATTCATGGTTTACTGATTTCAGTCGAATTGATATTTTAGTTCAAAGTCAAAGATTA
GGGCCAATGGATTTGTCAAAAACTCCTTTTAAGGTTTGTTAATGAGAGCTAAAGCTTTAA
TAGGAGCAAGTAACAGATTAAGGTAAACGCTTTCTACAGAAACCTAGACTGTTTAATGGT
TCACATTCGTTTCAGTTTTCGTAAGTTTGGTAGTTGTGCGAGTTTATTAACTTAATAATT
GACATTAATTAGTTTAAGTATGGAGATCCGAGTTTGTGCCGCACTACTAATTTGCTACTT
AAAACCATTTTAAAACAGCCGACCAAAAGTAAAACCATTGGAAATAACAAGAAGCTTCGT
TTGATGAGAGAAAACGTTATTAAAAAAGAAATCTACATGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 04-K108066-0022-153-H11C-8409
GenBank Accession LN487949 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K108066-0022-153-H11C-8409
CATTTACTGTAGATTTCTTTTTAATAACGTTTTCTCTCATCAAACGACCCTTCTTGTTAT
TTCCAATGGTTTTACTTTTGGTCGGCTGTTTTATAATGGTTTTAAGTAGCAAATTAGTAG
TGCGGCACAAACTCGGATCTCCATACTTAAACTAATTAATGTCAATTATTAAGTTAATAA
ACTCGCACAACTACCAAACTTACGAAAACTGAAACGAATGTGAACCATTAAACAGTCTAG
GTTTCTGTAGAAAGCGTTTACCTTAATCTGTTACTTGCTCCTATTAAAGCTTTAGCTCTC
ATTAACAAACCTTAAAAGGAGTTTTTGACAAATCCATTGGCCCTAATCTTTGACTTTGAA
CTAAAATATCAATTCGACTGAAATCAGTAAACCATGAATATCAATTACGTACACACTAAA
CTAATCATCAATTTGCCTATTTCACTACCTTTTTAAATCATTCTAACCCAAAATCATTTA
TAATCAAACCAATCTAAGCAAAATCCCATTTCACAAAAATAACCAAATCCAAACAAAACT
TTACTTACTTGGTGAAAACCGATCTCTTTA

Last Updated on 06.12.2018 16:11