Sequences from confirmation amplicon


Confirmation sequences for line 154F03 and hit At3g14205 are shown.
Line ID 154F03
Locus At3g14205 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4718894
Primers used for PCR 06qr - 8474, show primer details
   
Sequence Name 91-K115779-0022-154-F03C-06qr
GenBank Accession LN515138 [GenBank]
Sequencing primer gene specific primer, 06qr: 5'-TCATGCATACACTTCAGAGGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K115779-0022-154-F03C-06qr
TTACACGAACGATGTTTGTCTGGAAGAATACTTAACTCGAAGGATTCGGAATAACGTGAA
GGACTGTATGTGGACAGTTGCCTTGGTATATGGATTTTTCAAACAGGTATCACTTGTACT
AATTTGTCCAAAGGTTCTATTAATTTTATCTAACATTCAGAGATATGCATATTACAAACA
GGTGTTTAGATATTTTAACCTCACATTGAAAATTCCGTGTCCATGTTGCTTGATTTTGAA
AATTTGTACAGCATGAGAGAAGAAGCTCTCATAAGAATTAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCACAGCGTTAT
T
   
Sequence Name 43-K115778-0022-154-F03C-8409
GenBank Accession LN504952 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K115778-0022-154-F03C-8409
ATTTACATTGAATAATCCTAATTCTTATGAGAGCTTCTTCTCTCATGCTGTACAAATTTT
CAAAATCAAGCAACATGGACACGGAATTTTCAATGTGAGGTTAAAATATCTAAACACCTG
TTTGTAATATGCATATCTCTGAATGTTAGATAAAATTAATAGAACCTTTGGACAAATTAG
TACAAGTGATACCTGTTTGAAAAATCCATATACCAAGGCAACTGTCCACATACAGTCCTT
CACGTTATTCCGAATCCTTCGAGTTAAGTATTCGTTCCAGACAAACATCGATTCGTAGTA
AGTGTGTCCTTCCACATTGTTAGACAGATTCCTCTGAAGTGTATGCATG

Last Updated on 06.12.2018 16:11