Sequences from confirmation amplicon


Confirmation sequences for line 154H10 and hit At3g57330 are shown.
Line ID 154H10
Locus At3g57330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21214386
Primers used for PCR RK35 - 8474, show primer details
   
Sequence Name 41-K015902-022-154-H10C-8409
GenBank Accession LN503888 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K015902-022-154-H10C-8409
GTAGATTTCCCGGACATGAAGCCATTTCAGAGATTTGGATCGAGAGAAAAAGAAGATTAT
TATTCAAGTCACCAGGGATGGCAGCAGACAAGAGGTCTCCATCCATGACTTGGTTGTTGG
AGATGTGGTCCATTTATCTATTGGGGATCAAGTTCCAGCTGATGGGATTTTTATATCCGG
ATACAATCTGGAGATAGACGAGTCAAGCTTATCGGGCGAGAGTGAACCATCACATGTGAA
TAAAGAGAAGCCGTTTCTGCTTTCAGGAACCAAAGTCCAAAACGGCTCTGCAAAAATGCT
GGTGACAACCGTTGGTATGAGGACTGAGTGGGGAAAGTTGATGGATACTCTGAGTGAGGG
TGGAGAGGATGAGACCCCTCTGCAGGTGAAACTAAATGGTGTTGCAACAATCATAGGTAA
GATTGGTTTAGGCTTTGCAGTGCTTACGTTTGTGGTGTTGTGCATAAGGTTCGTTGTGGA
GAAAGCCACGGCTGGTAGCATCACTGAGTGGTCTTCTGAAGATGCACTAACATTGCTTGA
CTACTTTGCAATTGCTGTTACCATT
   
Sequence Name 42-K015902-022-154-H10C-RK35
GenBank Accession LN504278 [GenBank]
Sequencing primer gene specific primer, RK35: 5'-GCAACGACTATAATGGTAACAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K015902-022-154-H10C-rk35
ATTGCAAAGTAGTCAAGCAATGTTAGTGCATCTTCAGAAGACCACTCAGTGATGCTACCA
GCCGTGGCTTTCTCCACAACGAACCTTATGCACAACACCACAAACGTAAGCACTGCAAAG
CCTAAACCAATCTTACCTATGATTGTTGCAACACCATTTAGTTTCACCTGCAGAGGGGTC
TCATCCTCTCCACCCTCACTCAGAGTATCCATCAACTTTCCCCACTCAGTCCTCATACCA
ACGGTTGTCACCAGCATTTTTGCAGAGCCGTTTTGGACTTTGGTTCCTGAAAGCAGAAAC
GGCTTCTCTTTATTCACATGTGATGGTTCACTCTCGCCCGATAAGCTTGACTCGTCTATC
TCCAGATTGTATCCGGATATAAAAATCCCATCAGCTGGAACTTGATCCCCAATAGATAAA
TGGACCACATCTCCAACAACCAAGTCATGGATGGAGACCTCTTGTCTGCTGCCATCCCTG
GTGACTTGAATAATAATCTTCTTTTTCTCTCGATCCAAATCTCTGAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11